Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07760 and SGL_RS07765 are separated by 8 nucleotides SGL_RS07765 and SGL_RS07770 are separated by 302 nucleotides SGL_RS07770 and SGL_RS07775 are separated by 55 nucleotides
SGL_RS07760: SGL_RS07760 - pitrilysin family protein, at 1,258,444 to 1,259,751
_RS07760
SGL_RS07765: SGL_RS07765 - pitrilysin family protein, at 1,259,760 to 1,261,052
_RS07765
SGL_RS07770: SGL_RS07770 - HupE/UreJ family protein, at 1,261,355 to 1,261,993
_RS07770
SGL_RS07775: SGL_RS07775 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase, at 1,262,049 to 1,263,260
_RS07775
Position (kb)
1259
1260
1261
1262 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 1259.054 kb on - strand, within SGL_RS07760 at 1259.103 kb on + strand, within SGL_RS07760 at 1259.286 kb on + strand, within SGL_RS07760 at 1259.310 kb on + strand, within SGL_RS07760 at 1259.318 kb on - strand, within SGL_RS07760 at 1259.494 kb on + strand, within SGL_RS07760 at 1259.545 kb on + strand, within SGL_RS07760 at 1259.545 kb on + strand, within SGL_RS07760 at 1259.545 kb on + strand, within SGL_RS07760 at 1259.545 kb on + strand, within SGL_RS07760 at 1259.553 kb on - strand, within SGL_RS07760 at 1259.595 kb on - strand, within SGL_RS07760 at 1259.610 kb on + strand, within SGL_RS07760 at 1259.695 kb on + strand at 1259.700 kb on + strand at 1259.747 kb on + strand at 1259.842 kb on + strand at 1259.959 kb on - strand, within SGL_RS07765 at 1259.993 kb on + strand, within SGL_RS07765 at 1260.000 kb on + strand, within SGL_RS07765 at 1260.001 kb on - strand, within SGL_RS07765 at 1260.361 kb on + strand, within SGL_RS07765 at 1260.528 kb on + strand, within SGL_RS07765 at 1260.536 kb on - strand, within SGL_RS07765 at 1260.909 kb on - strand, within SGL_RS07765 at 1260.921 kb on + strand, within SGL_RS07765 at 1261.043 kb on - strand at 1261.056 kb on + strand at 1261.078 kb on - strand at 1261.177 kb on - strand at 1261.191 kb on - strand at 1261.256 kb on + strand at 1261.295 kb on + strand at 1261.323 kb on + strand at 1261.378 kb on - strand at 1261.631 kb on - strand, within SGL_RS07770 at 1261.667 kb on - strand, within SGL_RS07770 at 1261.757 kb on + strand, within SGL_RS07770 at 1261.759 kb on + strand, within SGL_RS07770 at 1261.874 kb on + strand, within SGL_RS07770 at 1261.891 kb on + strand, within SGL_RS07770 at 1261.914 kb on - strand, within SGL_RS07770 at 1261.964 kb on + strand at 1261.972 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,259,054 - SGL_RS07760 0.47 -2.0 1,259,103 + SGL_RS07760 0.50 -1.6 1,259,286 + SGL_RS07760 0.64 -1.7 1,259,310 + SGL_RS07760 0.66 -1.1 1,259,318 - SGL_RS07760 0.67 -1.4 1,259,494 + SGL_RS07760 0.80 -1.6 1,259,545 + SGL_RS07760 0.84 -2.0 1,259,545 + SGL_RS07760 0.84 -2.1 1,259,545 + SGL_RS07760 0.84 -1.5 1,259,545 + SGL_RS07760 0.84 -1.2 1,259,553 - SGL_RS07760 0.85 +0.4 1,259,595 - SGL_RS07760 0.88 -2.1 1,259,610 + SGL_RS07760 0.89 -1.6 1,259,695 + -1.2 1,259,700 + -1.6 1,259,747 + +0.1 1,259,842 + -0.4 1,259,959 - SGL_RS07765 0.15 -2.1 1,259,993 + SGL_RS07765 0.18 -1.9 1,260,000 + SGL_RS07765 0.19 -2.3 1,260,001 - SGL_RS07765 0.19 -1.4 1,260,361 + SGL_RS07765 0.46 -2.1 1,260,528 + SGL_RS07765 0.59 -2.0 1,260,536 - SGL_RS07765 0.60 -0.8 1,260,909 - SGL_RS07765 0.89 -1.7 1,260,921 + SGL_RS07765 0.90 -2.3 1,261,043 - -0.5 1,261,056 + -1.7 1,261,078 - -1.9 1,261,177 - -0.1 1,261,191 - +0.5 1,261,256 + -3.1 1,261,295 + -5.9 1,261,323 + -6.8 1,261,378 - -5.8 1,261,631 - SGL_RS07770 0.43 -6.8 1,261,667 - SGL_RS07770 0.49 -6.0 1,261,757 + SGL_RS07770 0.63 -5.9 1,261,759 + SGL_RS07770 0.63 -7.8 1,261,874 + SGL_RS07770 0.81 -5.1 1,261,891 + SGL_RS07770 0.84 -6.9 1,261,914 - SGL_RS07770 0.87 -7.5 1,261,964 + -6.7 1,261,972 - -4.2
Or see this region's nucleotide sequence