Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07485 and SGL_RS07490 are separated by 26 nucleotides SGL_RS07490 and SGL_RS07495 are separated by 51 nucleotides SGL_RS07495 and SGL_RS07500 are separated by 198 nucleotides SGL_RS07500 and SGL_RS07505 are separated by 12 nucleotides
SGL_RS07485: SGL_RS07485 - anthranilate phosphoribosyltransferase, at 1,206,947 to 1,207,993
_RS07485
SGL_RS07490: SGL_RS07490 - deoxyribose-phosphate aldolase, at 1,208,020 to 1,208,697
_RS07490
SGL_RS07495: SGL_RS07495 - GH116 family glycosyl hydrolase, at 1,208,749 to 1,211,202
_RS07495
SGL_RS07500: SGL_RS07500 - UPF0175 family protein, at 1,211,401 to 1,211,625
_RS07500
SGL_RS07505: SGL_RS07505 - Rpn family recombination-promoting nuclease/putative transposase, at 1,211,638 to 1,212,420
_RS07505
Position (kb)
1208
1209
1210
1211
1212 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1208.114 kb on + strand, within SGL_RS07490 at 1208.145 kb on + strand, within SGL_RS07490 at 1208.158 kb on - strand, within SGL_RS07490 at 1208.193 kb on + strand, within SGL_RS07490 at 1208.198 kb on - strand, within SGL_RS07490 at 1208.236 kb on + strand, within SGL_RS07490 at 1208.314 kb on + strand, within SGL_RS07490 at 1208.336 kb on - strand, within SGL_RS07490 at 1208.452 kb on - strand, within SGL_RS07490 at 1208.562 kb on + strand, within SGL_RS07490 at 1208.590 kb on + strand, within SGL_RS07490 at 1208.615 kb on + strand, within SGL_RS07490 at 1208.622 kb on + strand, within SGL_RS07490 at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.641 kb on + strand at 1208.648 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.649 kb on - strand at 1208.900 kb on - strand at 1209.096 kb on - strand, within SGL_RS07495 at 1209.122 kb on + strand, within SGL_RS07495 at 1209.152 kb on + strand, within SGL_RS07495 at 1209.231 kb on + strand, within SGL_RS07495 at 1209.301 kb on + strand, within SGL_RS07495 at 1209.422 kb on - strand, within SGL_RS07495 at 1209.458 kb on - strand, within SGL_RS07495 at 1209.482 kb on - strand, within SGL_RS07495 at 1209.547 kb on + strand, within SGL_RS07495 at 1209.555 kb on - strand, within SGL_RS07495 at 1209.566 kb on - strand, within SGL_RS07495 at 1209.666 kb on + strand, within SGL_RS07495 at 1209.666 kb on + strand, within SGL_RS07495 at 1209.686 kb on + strand, within SGL_RS07495 at 1209.847 kb on + strand, within SGL_RS07495 at 1209.881 kb on - strand, within SGL_RS07495 at 1209.946 kb on + strand, within SGL_RS07495 at 1209.985 kb on - strand, within SGL_RS07495 at 1210.100 kb on + strand, within SGL_RS07495 at 1210.119 kb on - strand, within SGL_RS07495 at 1210.408 kb on + strand, within SGL_RS07495 at 1210.478 kb on + strand, within SGL_RS07495 at 1210.483 kb on + strand, within SGL_RS07495 at 1210.553 kb on - strand, within SGL_RS07495 at 1210.649 kb on - strand, within SGL_RS07495 at 1210.654 kb on + strand, within SGL_RS07495 at 1210.707 kb on - strand, within SGL_RS07495 at 1210.707 kb on - strand, within SGL_RS07495 at 1210.707 kb on - strand, within SGL_RS07495 at 1210.817 kb on + strand, within SGL_RS07495 at 1210.841 kb on - strand, within SGL_RS07495 at 1210.901 kb on - strand, within SGL_RS07495 at 1210.915 kb on + strand, within SGL_RS07495 at 1211.090 kb on - strand at 1211.094 kb on + strand at 1211.102 kb on - strand at 1211.120 kb on + strand at 1211.120 kb on + strand at 1211.120 kb on + strand at 1211.128 kb on - strand at 1211.128 kb on - strand at 1211.200 kb on - strand at 1211.224 kb on + strand at 1211.282 kb on + strand at 1211.282 kb on + strand at 1211.293 kb on + strand at 1211.301 kb on - strand at 1211.550 kb on - strand, within SGL_RS07500
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,208,114 + SGL_RS07490 0.14 +0.6 1,208,145 + SGL_RS07490 0.18 -0.5 1,208,158 - SGL_RS07490 0.20 -0.3 1,208,193 + SGL_RS07490 0.26 -0.1 1,208,198 - SGL_RS07490 0.26 -0.6 1,208,236 + SGL_RS07490 0.32 -0.7 1,208,314 + SGL_RS07490 0.43 -0.5 1,208,336 - SGL_RS07490 0.47 -0.6 1,208,452 - SGL_RS07490 0.64 -0.3 1,208,562 + SGL_RS07490 0.80 -0.9 1,208,590 + SGL_RS07490 0.84 -0.1 1,208,615 + SGL_RS07490 0.88 -0.6 1,208,622 + SGL_RS07490 0.89 -0.1 1,208,641 + -0.3 1,208,641 + -0.7 1,208,641 + -0.6 1,208,641 + -0.3 1,208,641 + -0.6 1,208,641 + -0.9 1,208,641 + -0.4 1,208,641 + -0.6 1,208,648 - -0.8 1,208,649 - -0.0 1,208,649 - -0.7 1,208,649 - +0.1 1,208,649 - -0.6 1,208,649 - -0.9 1,208,649 - -0.4 1,208,649 - -0.5 1,208,649 - -0.8 1,208,649 - -0.3 1,208,900 - -4.2 1,209,096 - SGL_RS07495 0.14 -5.7 1,209,122 + SGL_RS07495 0.15 -6.5 1,209,152 + SGL_RS07495 0.16 -7.3 1,209,231 + SGL_RS07495 0.20 -6.2 1,209,301 + SGL_RS07495 0.22 -6.3 1,209,422 - SGL_RS07495 0.27 -7.4 1,209,458 - SGL_RS07495 0.29 -4.8 1,209,482 - SGL_RS07495 0.30 -4.0 1,209,547 + SGL_RS07495 0.33 -2.2 1,209,555 - SGL_RS07495 0.33 -3.1 1,209,566 - SGL_RS07495 0.33 -4.0 1,209,666 + SGL_RS07495 0.37 -4.9 1,209,666 + SGL_RS07495 0.37 -3.5 1,209,686 + SGL_RS07495 0.38 -5.6 1,209,847 + SGL_RS07495 0.45 -7.4 1,209,881 - SGL_RS07495 0.46 -6.1 1,209,946 + SGL_RS07495 0.49 -5.2 1,209,985 - SGL_RS07495 0.50 -6.0 1,210,100 + SGL_RS07495 0.55 -4.2 1,210,119 - SGL_RS07495 0.56 -6.0 1,210,408 + SGL_RS07495 0.68 -6.3 1,210,478 + SGL_RS07495 0.70 -6.7 1,210,483 + SGL_RS07495 0.71 -5.6 1,210,553 - SGL_RS07495 0.74 -4.9 1,210,649 - SGL_RS07495 0.77 -3.8 1,210,654 + SGL_RS07495 0.78 -5.6 1,210,707 - SGL_RS07495 0.80 -5.5 1,210,707 - SGL_RS07495 0.80 -4.1 1,210,707 - SGL_RS07495 0.80 -5.7 1,210,817 + SGL_RS07495 0.84 -7.3 1,210,841 - SGL_RS07495 0.85 -2.9 1,210,901 - SGL_RS07495 0.88 -6.2 1,210,915 + SGL_RS07495 0.88 -5.4 1,211,090 - -4.8 1,211,094 + -4.3 1,211,102 - -6.4 1,211,120 + -4.5 1,211,120 + -5.0 1,211,120 + -6.8 1,211,128 - -6.1 1,211,128 - -7.6 1,211,200 - -4.6 1,211,224 + -3.9 1,211,282 + -0.8 1,211,282 + +0.2 1,211,293 + +0.4 1,211,301 - +0.0 1,211,550 - SGL_RS07500 0.66 -0.3
Or see this region's nucleotide sequence