Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06970 and SGL_RS06975 are separated by 30 nucleotides SGL_RS06975 and SGL_RS06980 are separated by 35 nucleotides SGL_RS06980 and SGL_RS06985 are separated by 153 nucleotides SGL_RS06985 and SGL_RS06990 are separated by 104 nucleotides
SGL_RS06970: SGL_RS06970 - pentapeptide repeat-containing protein, at 1,091,698 to 1,092,894
_RS06970
SGL_RS06975: SGL_RS06975 - HAD-IA family hydrolase, at 1,092,925 to 1,093,590
_RS06975
SGL_RS06980: SGL_RS06980 - tRNA epoxyqueuosine(34) reductase QueG, at 1,093,626 to 1,094,495
_RS06980
SGL_RS06985: SGL_RS06985 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,094,649 to 1,095,143
_RS06985
SGL_RS06990: SGL_RS06990 - photosystem II reaction center protein PsbZ, at 1,095,248 to 1,095,436
_RS06990
Position (kb)
1093
1094
1095 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1092.632 kb on - strand, within SGL_RS06970 at 1092.755 kb on - strand, within SGL_RS06970 at 1092.779 kb on - strand at 1092.789 kb on - strand at 1092.800 kb on - strand at 1092.802 kb on + strand at 1092.903 kb on - strand at 1092.924 kb on - strand at 1092.991 kb on - strand at 1093.444 kb on + strand, within SGL_RS06975 at 1093.674 kb on - strand at 1093.706 kb on - strand at 1093.804 kb on - strand, within SGL_RS06980 at 1093.982 kb on - strand, within SGL_RS06980 at 1094.033 kb on - strand, within SGL_RS06980 at 1094.132 kb on - strand, within SGL_RS06980 at 1094.294 kb on + strand, within SGL_RS06980 at 1094.326 kb on + strand, within SGL_RS06980 at 1094.445 kb on + strand at 1094.627 kb on + strand at 1094.645 kb on + strand at 1094.668 kb on + strand at 1094.800 kb on + strand, within SGL_RS06985 at 1095.202 kb on - strand at 1095.204 kb on + strand at 1095.212 kb on - strand at 1095.263 kb on + strand at 1095.349 kb on - strand, within SGL_RS06990 at 1095.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,092,632 - SGL_RS06970 0.78 -0.2 1,092,755 - SGL_RS06970 0.88 -0.2 1,092,779 - -0.2 1,092,789 - -0.5 1,092,800 - -0.1 1,092,802 + -0.4 1,092,903 - +0.3 1,092,924 - +0.0 1,092,991 - -1.0 1,093,444 + SGL_RS06975 0.78 -1.8 1,093,674 - -1.6 1,093,706 - -3.0 1,093,804 - SGL_RS06980 0.20 -1.3 1,093,982 - SGL_RS06980 0.41 -1.8 1,094,033 - SGL_RS06980 0.47 -1.6 1,094,132 - SGL_RS06980 0.58 -1.4 1,094,294 + SGL_RS06980 0.77 -1.7 1,094,326 + SGL_RS06980 0.80 -3.0 1,094,445 + -1.9 1,094,627 + -1.0 1,094,645 + -1.2 1,094,668 + -3.2 1,094,800 + SGL_RS06985 0.31 -3.4 1,095,202 - -4.8 1,095,204 + -3.2 1,095,212 - -1.4 1,095,263 + -2.0 1,095,349 - SGL_RS06990 0.53 -1.6 1,095,444 - -1.6
Or see this region's nucleotide sequence