Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06785 and SGL_RS06790 are separated by 52 nucleotides SGL_RS06790 and SGL_RS06795 are separated by 82 nucleotides SGL_RS06795 and SGL_RS06800 are separated by 80 nucleotides SGL_RS06800 and SGL_RS06805 are separated by 396 nucleotides SGL_RS06805 and SGL_RS06810 are separated by 142 nucleotides
SGL_RS06785: SGL_RS06785 - alpha/beta hydrolase, at 1,049,517 to 1,050,407
_RS06785
SGL_RS06790: SGL_RS06790 - hypothetical protein, at 1,050,460 to 1,050,729
_RS06790
SGL_RS06795: SGL_RS06795 - glutaredoxin 3, at 1,050,812 to 1,051,078
_RS06795
SGL_RS06800: SGL_RS06800 - glutathione synthase, at 1,051,159 to 1,052,121
_RS06800
SGL_RS06805: SGL_RS06805 - hypothetical protein, at 1,052,518 to 1,052,784
_RS06805
SGL_RS06810: SGL_RS06810 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 1,052,927 to 1,053,754
_RS06810
Position (kb) 1051
1052
1053 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1050.252 kb on + strand, within SGL_RS06785 at 1050.263 kb on + strand, within SGL_RS06785 at 1050.302 kb on + strand, within SGL_RS06785 at 1050.389 kb on + strand at 1050.390 kb on + strand at 1050.397 kb on - strand at 1050.397 kb on - strand at 1050.398 kb on - strand at 1050.409 kb on + strand at 1050.409 kb on - strand at 1050.645 kb on + strand, within SGL_RS06790 at 1050.733 kb on - strand at 1051.037 kb on - strand, within SGL_RS06795 at 1051.082 kb on + strand at 1051.146 kb on + strand at 1051.217 kb on - strand at 1051.310 kb on - strand, within SGL_RS06800 at 1051.374 kb on + strand, within SGL_RS06800 at 1051.389 kb on + strand, within SGL_RS06800 at 1051.389 kb on + strand, within SGL_RS06800 at 1051.408 kb on + strand, within SGL_RS06800 at 1051.437 kb on - strand, within SGL_RS06800 at 1051.542 kb on - strand, within SGL_RS06800 at 1051.641 kb on + strand, within SGL_RS06800 at 1051.737 kb on + strand, within SGL_RS06800 at 1052.064 kb on + strand at 1052.699 kb on - strand, within SGL_RS06805 at 1052.778 kb on + strand at 1052.778 kb on + strand at 1052.778 kb on + strand at 1052.786 kb on - strand at 1052.809 kb on + strand at 1052.925 kb on + strand at 1053.093 kb on - strand, within SGL_RS06810
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 1,050,252 + SGL_RS06785 0.82 -0.6 1,050,263 + SGL_RS06785 0.84 +0.1 1,050,302 + SGL_RS06785 0.88 -0.4 1,050,389 + -0.8 1,050,390 + -0.1 1,050,397 - +0.3 1,050,397 - -0.1 1,050,398 - -0.3 1,050,409 + -0.2 1,050,409 - -0.0 1,050,645 + SGL_RS06790 0.69 +0.3 1,050,733 - -0.3 1,051,037 - SGL_RS06795 0.84 -0.8 1,051,082 + -0.4 1,051,146 + -2.7 1,051,217 - -3.0 1,051,310 - SGL_RS06800 0.16 -3.9 1,051,374 + SGL_RS06800 0.22 -5.8 1,051,389 + SGL_RS06800 0.24 -4.8 1,051,389 + SGL_RS06800 0.24 -2.3 1,051,408 + SGL_RS06800 0.26 -3.6 1,051,437 - SGL_RS06800 0.29 -6.6 1,051,542 - SGL_RS06800 0.40 -6.3 1,051,641 + SGL_RS06800 0.50 -5.4 1,051,737 + SGL_RS06800 0.60 -5.2 1,052,064 + -5.6 1,052,699 - SGL_RS06805 0.68 -0.9 1,052,778 + +0.5 1,052,778 + -1.0 1,052,778 + -0.7 1,052,786 - +0.2 1,052,809 + +0.8 1,052,925 + -0.1 1,053,093 - SGL_RS06810 0.20 -1.6
Or see this region's nucleotide sequence