Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04345 and SGL_RS04350 overlap by 4 nucleotides SGL_RS04350 and SGL_RS04355 overlap by 4 nucleotides SGL_RS04355 and SGL_RS04360 are separated by 267 nucleotides SGL_RS04360 and SGL_RS04365 are separated by 156 nucleotides
SGL_RS04345: SGL_RS04345 - response regulator, at 533,113 to 537,474
_RS04345
SGL_RS04350: SGL_RS04350 - PleD family two-component system response regulator, at 537,471 to 538,418
_RS04350
SGL_RS04355: SGL_RS04355 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 538,415 to 539,020
_RS04355
SGL_RS04360: SGL_RS04360 - 5-formyltetrahydrofolate cyclo-ligase, at 539,288 to 539,854
_RS04360
SGL_RS04365: SGL_RS04365 - HAD family hydrolase, at 540,011 to 540,763
_RS04365
Position (kb)
538
539
540 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 537.418 kb on + strand at 537.444 kb on - strand at 537.455 kb on + strand at 537.458 kb on + strand at 537.487 kb on - strand at 537.588 kb on - strand, within SGL_RS04350 at 538.058 kb on + strand, within SGL_RS04350 at 538.087 kb on + strand, within SGL_RS04350 at 538.107 kb on + strand, within SGL_RS04350 at 538.119 kb on + strand, within SGL_RS04350 at 538.120 kb on - strand, within SGL_RS04350 at 538.211 kb on - strand, within SGL_RS04350 at 538.233 kb on + strand, within SGL_RS04350 at 538.249 kb on + strand, within SGL_RS04350 at 538.383 kb on + strand at 538.383 kb on + strand at 538.391 kb on - strand at 538.391 kb on - strand at 538.436 kb on - strand at 538.443 kb on + strand at 538.448 kb on + strand at 538.456 kb on - strand at 538.507 kb on - strand, within SGL_RS04355 at 538.587 kb on - strand, within SGL_RS04355 at 538.608 kb on - strand, within SGL_RS04355 at 538.776 kb on - strand, within SGL_RS04355 at 538.781 kb on - strand, within SGL_RS04355 at 538.802 kb on - strand, within SGL_RS04355 at 538.869 kb on - strand, within SGL_RS04355 at 538.890 kb on + strand, within SGL_RS04355 at 538.961 kb on + strand at 538.970 kb on - strand at 539.061 kb on - strand at 539.120 kb on - strand at 539.215 kb on + strand at 539.348 kb on - strand, within SGL_RS04360 at 539.545 kb on + strand, within SGL_RS04360 at 539.557 kb on + strand, within SGL_RS04360 at 539.570 kb on + strand, within SGL_RS04360 at 539.587 kb on - strand, within SGL_RS04360 at 539.601 kb on + strand, within SGL_RS04360 at 539.706 kb on - strand, within SGL_RS04360 at 539.884 kb on + strand at 539.890 kb on - strand at 539.892 kb on - strand at 539.894 kb on + strand at 539.894 kb on + strand at 539.932 kb on + strand at 539.981 kb on - strand at 539.983 kb on - strand at 539.983 kb on - strand at 539.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 537,418 + -0.4 537,444 - -0.9 537,455 + +0.5 537,458 + -0.0 537,487 - -0.1 537,588 - SGL_RS04350 0.12 -0.3 538,058 + SGL_RS04350 0.62 +0.4 538,087 + SGL_RS04350 0.65 -0.5 538,107 + SGL_RS04350 0.67 +0.1 538,119 + SGL_RS04350 0.68 +0.2 538,120 - SGL_RS04350 0.68 -0.2 538,211 - SGL_RS04350 0.78 -0.0 538,233 + SGL_RS04350 0.80 +0.0 538,249 + SGL_RS04350 0.82 -0.1 538,383 + -0.4 538,383 + -0.2 538,391 - +0.1 538,391 - -0.0 538,436 - +0.1 538,443 + -1.0 538,448 + -0.7 538,456 - -0.7 538,507 - SGL_RS04355 0.15 -1.3 538,587 - SGL_RS04355 0.28 -1.8 538,608 - SGL_RS04355 0.32 -0.1 538,776 - SGL_RS04355 0.60 -1.7 538,781 - SGL_RS04355 0.60 -1.2 538,802 - SGL_RS04355 0.64 -2.3 538,869 - SGL_RS04355 0.75 -2.2 538,890 + SGL_RS04355 0.78 -2.4 538,961 + -1.3 538,970 - -1.2 539,061 - -1.6 539,120 - -2.1 539,215 + -2.2 539,348 - SGL_RS04360 0.11 -2.4 539,545 + SGL_RS04360 0.45 -1.8 539,557 + SGL_RS04360 0.47 -1.7 539,570 + SGL_RS04360 0.50 -1.2 539,587 - SGL_RS04360 0.53 -1.6 539,601 + SGL_RS04360 0.55 -1.8 539,706 - SGL_RS04360 0.74 -2.8 539,884 + +0.9 539,890 - -1.1 539,892 - -0.3 539,894 + +0.4 539,894 + +1.4 539,932 + -0.8 539,981 - -0.6 539,983 - -0.1 539,983 - -0.1 539,993 - -0.9
Or see this region's nucleotide sequence