Experiment: D-Sorbitol 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03540 and SGL_RS03545 overlap by 4 nucleotides SGL_RS03545 and SGL_RS03550 are separated by 0 nucleotides SGL_RS03550 and SGL_RS03555 are separated by 18 nucleotides SGL_RS03555 and SGL_RS03560 overlap by 4 nucleotides
SGL_RS03540: SGL_RS03540 - ABC transporter ATP-binding protein, at 352,263 to 353,555
_RS03540
SGL_RS03545: SGL_RS03545 - glucose-1-phosphate cytidylyltransferase, at 353,552 to 354,322
_RS03545
SGL_RS03550: SGL_RS03550 - CDP-glucose 4,6-dehydratase, at 354,323 to 355,405
_RS03550
SGL_RS03555: SGL_RS03555 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 355,424 to 355,993
_RS03555
SGL_RS03560: SGL_RS03560 - class I SAM-dependent methyltransferase, at 355,990 to 357,210
_RS03560
Position (kb) 354
355
356 Strain fitness (log2 ratio) -9
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 353.481 kb on + strand at 353.486 kb on + strand at 353.820 kb on - strand, within SGL_RS03545 at 354.014 kb on + strand, within SGL_RS03545 at 354.213 kb on - strand, within SGL_RS03545 at 354.409 kb on - strand at 354.459 kb on + strand, within SGL_RS03550 at 354.468 kb on - strand, within SGL_RS03550 at 354.504 kb on + strand, within SGL_RS03550 at 354.590 kb on + strand, within SGL_RS03550 at 354.602 kb on - strand, within SGL_RS03550 at 354.614 kb on - strand, within SGL_RS03550 at 354.615 kb on + strand, within SGL_RS03550 at 354.667 kb on - strand, within SGL_RS03550 at 354.760 kb on + strand, within SGL_RS03550 at 354.790 kb on + strand, within SGL_RS03550 at 354.869 kb on + strand, within SGL_RS03550 at 354.877 kb on - strand, within SGL_RS03550 at 355.078 kb on - strand, within SGL_RS03550 at 355.092 kb on - strand, within SGL_RS03550 at 355.217 kb on + strand, within SGL_RS03550 at 355.308 kb on + strand at 355.375 kb on + strand at 355.382 kb on - strand at 355.584 kb on + strand, within SGL_RS03555 at 355.584 kb on + strand, within SGL_RS03555 at 355.606 kb on - strand, within SGL_RS03555 at 355.740 kb on + strand, within SGL_RS03555 at 355.748 kb on - strand, within SGL_RS03555 at 355.765 kb on - strand, within SGL_RS03555 at 355.789 kb on - strand, within SGL_RS03555 at 355.831 kb on + strand, within SGL_RS03555 at 355.859 kb on - strand, within SGL_RS03555 at 355.909 kb on - strand, within SGL_RS03555 at 355.920 kb on - strand, within SGL_RS03555 at 356.014 kb on - strand at 356.210 kb on + strand, within SGL_RS03560 at 356.211 kb on + strand, within SGL_RS03560
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol 500 mM remove 353,481 + -5.0 353,486 + -5.2 353,820 - SGL_RS03545 0.35 -4.7 354,014 + SGL_RS03545 0.60 -6.2 354,213 - SGL_RS03545 0.86 -5.2 354,409 - -6.8 354,459 + SGL_RS03550 0.13 -6.1 354,468 - SGL_RS03550 0.13 -3.4 354,504 + SGL_RS03550 0.17 -5.7 354,590 + SGL_RS03550 0.25 -4.2 354,602 - SGL_RS03550 0.26 -7.1 354,614 - SGL_RS03550 0.27 -7.2 354,615 + SGL_RS03550 0.27 -7.5 354,667 - SGL_RS03550 0.32 -8.3 354,760 + SGL_RS03550 0.40 -8.2 354,790 + SGL_RS03550 0.43 -7.1 354,869 + SGL_RS03550 0.50 -9.0 354,877 - SGL_RS03550 0.51 -6.9 355,078 - SGL_RS03550 0.70 -7.3 355,092 - SGL_RS03550 0.71 -6.7 355,217 + SGL_RS03550 0.83 -7.5 355,308 + -7.4 355,375 + +0.3 355,382 - +0.4 355,584 + SGL_RS03555 0.28 -4.5 355,584 + SGL_RS03555 0.28 -4.2 355,606 - SGL_RS03555 0.32 -4.7 355,740 + SGL_RS03555 0.55 -5.2 355,748 - SGL_RS03555 0.57 -6.0 355,765 - SGL_RS03555 0.60 -4.1 355,789 - SGL_RS03555 0.64 -6.0 355,831 + SGL_RS03555 0.71 -4.8 355,859 - SGL_RS03555 0.76 -3.5 355,909 - SGL_RS03555 0.85 -3.7 355,920 - SGL_RS03555 0.87 -5.4 356,014 - -6.8 356,210 + SGL_RS03560 0.18 -4.3 356,211 + SGL_RS03560 0.18 -4.0
Or see this region's nucleotide sequence