Experiment: Chloride 800 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08105 and SGL_RS08110 are separated by 119 nucleotides SGL_RS08110 and SGL_RS08115 are separated by 49 nucleotides SGL_RS08115 and SGL_RS08120 are separated by 284 nucleotides
SGL_RS08105: SGL_RS08105 - hypothetical protein, at 1,329,388 to 1,329,990
_RS08105
SGL_RS08110: SGL_RS08110 - type 1 glutamine amidotransferase, at 1,330,110 to 1,330,880
_RS08110
SGL_RS08115: SGL_RS08115 - NAD(P)/FAD-dependent oxidoreductase, at 1,330,930 to 1,332,144
_RS08115
SGL_RS08120: SGL_RS08120 - N-acetylmuramoyl-L-alanine amidase, at 1,332,429 to 1,334,378
_RS08120
Position (kb)
1330
1331
1332
1333 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1329.937 kb on - strand at 1329.958 kb on + strand at 1330.884 kb on + strand at 1330.926 kb on + strand at 1330.980 kb on + strand at 1331.010 kb on + strand at 1331.028 kb on - strand at 1331.029 kb on - strand at 1331.029 kb on - strand at 1331.212 kb on + strand, within SGL_RS08115 at 1331.220 kb on - strand, within SGL_RS08115 at 1331.237 kb on - strand, within SGL_RS08115 at 1331.275 kb on - strand, within SGL_RS08115 at 1331.285 kb on + strand, within SGL_RS08115 at 1331.347 kb on - strand, within SGL_RS08115 at 1331.505 kb on - strand, within SGL_RS08115 at 1331.541 kb on - strand, within SGL_RS08115 at 1331.661 kb on - strand, within SGL_RS08115 at 1331.991 kb on - strand, within SGL_RS08115 at 1332.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 800 mM remove 1,329,937 - +0.2 1,329,958 + -0.2 1,330,884 + +0.4 1,330,926 + +0.3 1,330,980 + -2.7 1,331,010 + -3.4 1,331,028 - -0.6 1,331,029 - -2.8 1,331,029 - -2.6 1,331,212 + SGL_RS08115 0.23 -4.0 1,331,220 - SGL_RS08115 0.24 -2.8 1,331,237 - SGL_RS08115 0.25 -3.8 1,331,275 - SGL_RS08115 0.28 -2.9 1,331,285 + SGL_RS08115 0.29 -3.2 1,331,347 - SGL_RS08115 0.34 -4.0 1,331,505 - SGL_RS08115 0.47 -3.0 1,331,541 - SGL_RS08115 0.50 -4.4 1,331,661 - SGL_RS08115 0.60 -3.4 1,331,991 - SGL_RS08115 0.87 -2.0 1,332,208 + +0.3
Or see this region's nucleotide sequence