Experiment: Chloride 800 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04140 and SGL_RS04145 are separated by 102 nucleotides SGL_RS04145 and SGL_RS04150 overlap by 11 nucleotides SGL_RS04150 and SGL_RS04155 are separated by 129 nucleotides SGL_RS04155 and SGL_RS04160 are separated by 268 nucleotides
SGL_RS04140: SGL_RS04140 - hypothetical protein, at 474,789 to 475,007
_RS04140
SGL_RS04145: SGL_RS04145 - DUF86 domain-containing protein, at 475,110 to 475,445
_RS04145
SGL_RS04150: SGL_RS04150 - nucleotidyltransferase family protein, at 475,435 to 475,743
_RS04150
SGL_RS04155: SGL_RS04155 - reverse transcriptase domain-containing protein, at 475,873 to 477,399
_RS04155
SGL_RS04160: SGL_RS04160 - glutamate synthase large subunit, at 477,668 to 482,320
_RS04160
Position (kb) 475
476
477
478 Strain fitness (log2 ratio) -1
0
1 at 476.721 kb on - strand, within SGL_RS04155 at 476.737 kb on + strand, within SGL_RS04155 at 476.933 kb on + strand, within SGL_RS04155 at 476.954 kb on + strand, within SGL_RS04155 at 477.997 kb on - strand at 478.235 kb on - strand, within SGL_RS04160 at 478.236 kb on - strand, within SGL_RS04160 at 478.237 kb on - strand, within SGL_RS04160 at 478.323 kb on - strand, within SGL_RS04160 at 478.325 kb on - strand, within SGL_RS04160
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 800 mM remove 476,721 - SGL_RS04155 0.56 -1.5 476,737 + SGL_RS04155 0.57 -0.9 476,933 + SGL_RS04155 0.69 -0.8 476,954 + SGL_RS04155 0.71 -0.8 477,997 - -0.7 478,235 - SGL_RS04160 0.12 -0.3 478,236 - SGL_RS04160 0.12 -1.3 478,237 - SGL_RS04160 0.12 -0.5 478,323 - SGL_RS04160 0.14 -0.4 478,325 - SGL_RS04160 0.14 -0.1
Or see this region's nucleotide sequence