Experiment: Chloride 800 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02160 and SGL_RS02165 are separated by 30 nucleotides SGL_RS02165 and SGL_RS02170 are separated by 26 nucleotides SGL_RS02170 and SGL_RS02175 are separated by 8 nucleotides SGL_RS02175 and SGL_RS02180 overlap by 65 nucleotides SGL_RS02180 and SGL_RS02185 are separated by 268 nucleotides SGL_RS02185 and SGL_RS02190 are separated by 209 nucleotides
SGL_RS02160: SGL_RS02160 - GDSL-type esterase/lipase family protein, at 69,284 to 69,985
_RS02160
SGL_RS02165: SGL_RS02165 - hypothetical protein, at 70,016 to 70,393
_RS02165
SGL_RS02170: SGL_RS02170 - hypothetical protein, at 70,420 to 70,935
_RS02170
SGL_RS02175: SGL_RS02175 - UPF0182 family protein, at 70,944 to 74,042
_RS02175
SGL_RS02180: SGL_RS02180 - hypothetical protein, at 73,978 to 74,229
_RS02180
SGL_RS02185: SGL_RS02185 - PepSY domain-containing protein, at 74,498 to 74,785
_RS02185
SGL_RS02190: SGL_RS02190 - A24 family peptidase, at 74,995 to 75,804
_RS02190
Position (kb) 70
71
72
73
74
75 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 70.167 kb on + strand, within SGL_RS02165 at 70.167 kb on + strand, within SGL_RS02165 at 70.489 kb on - strand, within SGL_RS02170 at 70.774 kb on - strand, within SGL_RS02170 at 71.363 kb on - strand, within SGL_RS02175 at 71.488 kb on - strand, within SGL_RS02175 at 71.674 kb on + strand, within SGL_RS02175 at 72.115 kb on - strand, within SGL_RS02175 at 72.232 kb on - strand, within SGL_RS02175 at 72.279 kb on + strand, within SGL_RS02175 at 72.287 kb on - strand, within SGL_RS02175 at 72.486 kb on - strand, within SGL_RS02175 at 72.572 kb on + strand, within SGL_RS02175 at 72.631 kb on + strand, within SGL_RS02175 at 72.649 kb on - strand, within SGL_RS02175 at 72.682 kb on - strand, within SGL_RS02175 at 72.718 kb on - strand, within SGL_RS02175 at 72.765 kb on - strand, within SGL_RS02175 at 72.803 kb on - strand, within SGL_RS02175 at 72.982 kb on - strand, within SGL_RS02175 at 73.040 kb on + strand, within SGL_RS02175 at 73.157 kb on - strand, within SGL_RS02175 at 73.167 kb on - strand, within SGL_RS02175 at 73.306 kb on - strand, within SGL_RS02175 at 73.367 kb on + strand, within SGL_RS02175 at 73.389 kb on - strand, within SGL_RS02175 at 73.391 kb on - strand, within SGL_RS02175 at 73.413 kb on + strand, within SGL_RS02175 at 73.413 kb on + strand, within SGL_RS02175 at 73.421 kb on - strand, within SGL_RS02175 at 73.528 kb on + strand, within SGL_RS02175 at 73.541 kb on - strand, within SGL_RS02175 at 73.650 kb on + strand, within SGL_RS02175 at 73.685 kb on + strand, within SGL_RS02175 at 73.695 kb on + strand, within SGL_RS02175 at 73.721 kb on - strand, within SGL_RS02175 at 73.748 kb on + strand at 73.787 kb on + strand at 73.795 kb on - strand at 73.830 kb on + strand at 73.954 kb on + strand at 73.967 kb on - strand at 74.038 kb on - strand at 74.074 kb on - strand, within SGL_RS02180 at 74.107 kb on + strand, within SGL_RS02180 at 74.115 kb on - strand, within SGL_RS02180 at 74.115 kb on - strand, within SGL_RS02180 at 74.115 kb on - strand, within SGL_RS02180 at 74.374 kb on + strand at 74.416 kb on - strand at 74.543 kb on - strand, within SGL_RS02185 at 74.550 kb on + strand, within SGL_RS02185 at 74.644 kb on - strand, within SGL_RS02185 at 74.712 kb on + strand, within SGL_RS02185 at 74.799 kb on + strand at 74.949 kb on + strand at 74.960 kb on + strand at 75.037 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 800 mM remove 70,167 + SGL_RS02165 0.40 +0.0 70,167 + SGL_RS02165 0.40 -0.1 70,489 - SGL_RS02170 0.13 +0.1 70,774 - SGL_RS02170 0.69 -0.1 71,363 - SGL_RS02175 0.14 -1.5 71,488 - SGL_RS02175 0.18 -1.8 71,674 + SGL_RS02175 0.24 -2.2 72,115 - SGL_RS02175 0.38 -1.1 72,232 - SGL_RS02175 0.42 -1.8 72,279 + SGL_RS02175 0.43 -1.6 72,287 - SGL_RS02175 0.43 -1.4 72,486 - SGL_RS02175 0.50 -1.6 72,572 + SGL_RS02175 0.53 -1.7 72,631 + SGL_RS02175 0.54 -1.4 72,649 - SGL_RS02175 0.55 -2.3 72,682 - SGL_RS02175 0.56 -1.2 72,718 - SGL_RS02175 0.57 -0.9 72,765 - SGL_RS02175 0.59 -1.9 72,803 - SGL_RS02175 0.60 -1.0 72,982 - SGL_RS02175 0.66 -1.5 73,040 + SGL_RS02175 0.68 -1.2 73,157 - SGL_RS02175 0.71 -1.2 73,167 - SGL_RS02175 0.72 -0.7 73,306 - SGL_RS02175 0.76 -1.8 73,367 + SGL_RS02175 0.78 -1.7 73,389 - SGL_RS02175 0.79 -0.6 73,391 - SGL_RS02175 0.79 -0.7 73,413 + SGL_RS02175 0.80 -1.4 73,413 + SGL_RS02175 0.80 -2.2 73,421 - SGL_RS02175 0.80 -1.3 73,528 + SGL_RS02175 0.83 -1.4 73,541 - SGL_RS02175 0.84 -1.3 73,650 + SGL_RS02175 0.87 -1.8 73,685 + SGL_RS02175 0.88 -1.4 73,695 + SGL_RS02175 0.89 -0.5 73,721 - SGL_RS02175 0.90 -3.2 73,748 + -1.6 73,787 + -0.9 73,795 - -1.4 73,830 + -1.0 73,954 + -2.0 73,967 - -1.4 74,038 - -0.3 74,074 - SGL_RS02180 0.38 +0.4 74,107 + SGL_RS02180 0.51 -0.3 74,115 - SGL_RS02180 0.54 -0.0 74,115 - SGL_RS02180 0.54 -0.8 74,115 - SGL_RS02180 0.54 -0.1 74,374 + +0.5 74,416 - +0.0 74,543 - SGL_RS02185 0.16 -0.3 74,550 + SGL_RS02185 0.18 -0.0 74,644 - SGL_RS02185 0.51 +0.5 74,712 + SGL_RS02185 0.74 -0.2 74,799 + +0.0 74,949 + -0.2 74,960 + -0.0 75,037 + -1.7
Or see this region's nucleotide sequence