Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13540 and SGL_RS13545 are separated by 238 nucleotides SGL_RS13545 and SGL_RS13550 are separated by 258 nucleotides SGL_RS13550 and SGL_RS13555 are separated by 218 nucleotides
SGL_RS13540: SGL_RS13540 - hypothetical protein, at 2,509,162 to 2,509,584
_RS13540
SGL_RS13545: SGL_RS13545 - hypothetical protein, at 2,509,823 to 2,510,014
_RS13545
SGL_RS13550: SGL_RS13550 - long-chain acyl-[acyl-carrier-protein] reductase, at 2,510,273 to 2,511,295
_RS13550
SGL_RS13555: SGL_RS13555 - aldehyde oxygenase (deformylating), at 2,511,514 to 2,512,209
_RS13555
Position (kb)
2510
2511
2512 Strain fitness (log2 ratio)
-2
-1
0
1 at 2509.377 kb on - strand, within SGL_RS13540 at 2509.633 kb on + strand at 2509.734 kb on - strand at 2509.921 kb on + strand, within SGL_RS13545 at 2510.224 kb on + strand at 2510.262 kb on + strand at 2510.265 kb on - strand at 2510.662 kb on - strand, within SGL_RS13550 at 2511.038 kb on + strand, within SGL_RS13550 at 2511.230 kb on + strand at 2511.238 kb on - strand at 2511.358 kb on + strand at 2511.366 kb on - strand at 2511.405 kb on + strand at 2511.429 kb on + strand at 2511.652 kb on + strand, within SGL_RS13555 at 2511.788 kb on + strand, within SGL_RS13555 at 2512.136 kb on - strand, within SGL_RS13555 at 2512.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 2,509,377 - SGL_RS13540 0.51 +0.0 2,509,633 + -0.6 2,509,734 - +0.1 2,509,921 + SGL_RS13545 0.51 +0.2 2,510,224 + -0.2 2,510,262 + -0.7 2,510,265 - -0.1 2,510,662 - SGL_RS13550 0.38 -0.9 2,511,038 + SGL_RS13550 0.75 -1.2 2,511,230 + -0.7 2,511,238 - -1.3 2,511,358 + -1.4 2,511,366 - -0.9 2,511,405 + -0.7 2,511,429 + +0.2 2,511,652 + SGL_RS13555 0.20 -2.0 2,511,788 + SGL_RS13555 0.39 -0.6 2,512,136 - SGL_RS13555 0.89 -0.6 2,512,232 - -1.3
Or see this region's nucleotide sequence