Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00125 and SGL_RS18470 are separated by 112 nucleotides SGL_RS18470 and SGL_RS19675 are separated by 102 nucleotides SGL_RS19675 and SGL_RS00135 are separated by 213 nucleotides SGL_RS00135 and SGL_RS00140 overlap by 48 nucleotides
SGL_RS00125: SGL_RS00125 - DNA phosphorothioation-associated putative methyltransferase, at 28,096 to 30,168
_RS00125
SGL_RS18470: SGL_RS18470 - HNH endonuclease, at 30,281 to 30,574
_RS18470
SGL_RS19675: SGL_RS19675 - hypothetical protein, at 30,677 to 31,183
_RS19675
SGL_RS00135: SGL_RS00135 - IS701 family transposase, at 31,397 to 32,062
_RS00135
SGL_RS00140: SGL_RS00140 - ARMT1-like domain-containing protein, at 32,015 to 32,836
_RS00140
Position (kb) 30
31
32 Strain fitness (log2 ratio) -1
0
1 at 29.817 kb on - strand, within SGL_RS00125 at 29.957 kb on + strand, within SGL_RS00125 at 30.304 kb on + strand at 30.464 kb on - strand, within SGL_RS18470 at 30.486 kb on + strand, within SGL_RS18470 at 30.533 kb on + strand, within SGL_RS18470 at 30.642 kb on + strand at 30.661 kb on + strand at 30.679 kb on + strand at 30.800 kb on - strand, within SGL_RS19675 at 30.807 kb on - strand, within SGL_RS19675 at 30.838 kb on + strand, within SGL_RS19675 at 30.852 kb on - strand, within SGL_RS19675 at 30.957 kb on - strand, within SGL_RS19675 at 30.970 kb on + strand, within SGL_RS19675 at 30.978 kb on - strand, within SGL_RS19675 at 30.983 kb on - strand, within SGL_RS19675 at 31.005 kb on - strand, within SGL_RS19675 at 31.005 kb on - strand, within SGL_RS19675 at 31.006 kb on - strand, within SGL_RS19675 at 31.072 kb on - strand, within SGL_RS19675 at 31.147 kb on - strand at 31.216 kb on + strand at 31.269 kb on + strand at 31.277 kb on - strand at 31.277 kb on - strand at 31.279 kb on + strand at 31.287 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 29,817 - SGL_RS00125 0.83 -0.1 29,957 + SGL_RS00125 0.90 +0.3 30,304 + -0.1 30,464 - SGL_RS18470 0.62 +0.1 30,486 + SGL_RS18470 0.70 -0.2 30,533 + SGL_RS18470 0.86 +0.0 30,642 + -0.1 30,661 + +0.1 30,679 + +0.0 30,800 - SGL_RS19675 0.24 +0.1 30,807 - SGL_RS19675 0.26 -0.2 30,838 + SGL_RS19675 0.32 +0.5 30,852 - SGL_RS19675 0.35 -0.0 30,957 - SGL_RS19675 0.55 +0.1 30,970 + SGL_RS19675 0.58 +0.4 30,978 - SGL_RS19675 0.59 +0.8 30,983 - SGL_RS19675 0.60 +0.3 31,005 - SGL_RS19675 0.65 +0.2 31,005 - SGL_RS19675 0.65 +0.2 31,006 - SGL_RS19675 0.65 -0.1 31,072 - SGL_RS19675 0.78 +0.6 31,147 - +1.1 31,216 + +0.0 31,269 + +0.0 31,277 - -0.0 31,277 - -0.4 31,279 + +0.2 31,287 - +0.4
Or see this region's nucleotide sequence