Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12525 and SGL_RS12530 are separated by 203 nucleotides SGL_RS12530 and SGL_RS12535 are separated by 145 nucleotides SGL_RS12535 and SGL_RS12540 are separated by 40 nucleotides
SGL_RS12525: SGL_RS12525 - glycogen synthase GlgA, at 2,265,213 to 2,266,646
_RS12525
SGL_RS12530: SGL_RS12530 - DUF1830 domain-containing protein, at 2,266,850 to 2,267,344
_RS12530
SGL_RS12535: SGL_RS12535 - radical SAM protein, at 2,267,490 to 2,269,085
_RS12535
SGL_RS12540: SGL_RS12540 - RNA polymerase sigma factor, RpoD/SigA family, at 2,269,126 to 2,270,163
_RS12540
Position (kb)
2267
2268
2269
2270 Strain fitness (log2 ratio)
-1
0
1 at 2266.521 kb on + strand at 2266.540 kb on + strand at 2266.594 kb on + strand at 2266.792 kb on + strand at 2266.800 kb on - strand at 2266.897 kb on + strand at 2266.941 kb on + strand, within SGL_RS12530 at 2267.036 kb on + strand, within SGL_RS12530 at 2267.054 kb on + strand, within SGL_RS12530 at 2267.467 kb on + strand at 2267.469 kb on + strand at 2267.489 kb on - strand at 2267.892 kb on - strand, within SGL_RS12535 at 2267.987 kb on + strand, within SGL_RS12535 at 2268.038 kb on - strand, within SGL_RS12535 at 2268.091 kb on - strand, within SGL_RS12535 at 2268.172 kb on + strand, within SGL_RS12535 at 2268.277 kb on + strand, within SGL_RS12535 at 2268.299 kb on + strand, within SGL_RS12535 at 2268.448 kb on - strand, within SGL_RS12535 at 2268.450 kb on + strand, within SGL_RS12535 at 2268.458 kb on - strand, within SGL_RS12535 at 2268.458 kb on - strand, within SGL_RS12535 at 2268.619 kb on + strand, within SGL_RS12535 at 2268.852 kb on - strand, within SGL_RS12535 at 2268.917 kb on + strand, within SGL_RS12535 at 2268.998 kb on + strand at 2269.016 kb on - strand at 2269.016 kb on - strand at 2269.445 kb on - strand, within SGL_RS12540 at 2269.513 kb on - strand, within SGL_RS12540 at 2269.616 kb on + strand, within SGL_RS12540 at 2269.621 kb on + strand, within SGL_RS12540 at 2269.750 kb on - strand, within SGL_RS12540 at 2269.859 kb on - strand, within SGL_RS12540 at 2270.051 kb on - strand, within SGL_RS12540
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 2,266,521 + +0.8 2,266,540 + -0.3 2,266,594 + -0.3 2,266,792 + +0.2 2,266,800 - +0.1 2,266,897 + -0.5 2,266,941 + SGL_RS12530 0.18 +0.1 2,267,036 + SGL_RS12530 0.38 +0.3 2,267,054 + SGL_RS12530 0.41 -0.2 2,267,467 + -0.2 2,267,469 + -0.1 2,267,489 - +0.1 2,267,892 - SGL_RS12535 0.25 +0.5 2,267,987 + SGL_RS12535 0.31 +0.9 2,268,038 - SGL_RS12535 0.34 +0.0 2,268,091 - SGL_RS12535 0.38 +0.7 2,268,172 + SGL_RS12535 0.43 +0.4 2,268,277 + SGL_RS12535 0.49 -0.3 2,268,299 + SGL_RS12535 0.51 -0.3 2,268,448 - SGL_RS12535 0.60 -0.1 2,268,450 + SGL_RS12535 0.60 +1.0 2,268,458 - SGL_RS12535 0.61 +1.0 2,268,458 - SGL_RS12535 0.61 -0.3 2,268,619 + SGL_RS12535 0.71 -0.0 2,268,852 - SGL_RS12535 0.85 +0.7 2,268,917 + SGL_RS12535 0.89 +1.5 2,268,998 + -0.8 2,269,016 - -0.5 2,269,016 - +0.9 2,269,445 - SGL_RS12540 0.31 -0.5 2,269,513 - SGL_RS12540 0.37 -0.7 2,269,616 + SGL_RS12540 0.47 -0.6 2,269,621 + SGL_RS12540 0.48 -1.5 2,269,750 - SGL_RS12540 0.60 -1.5 2,269,859 - SGL_RS12540 0.71 -0.6 2,270,051 - SGL_RS12540 0.89 -0.3
Or see this region's nucleotide sequence