Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11610 and SGL_RS11615 are separated by 283 nucleotides SGL_RS11615 and SGL_RS11620 overlap by 15 nucleotides SGL_RS11620 and SGL_RS11625 are separated by 192 nucleotides
SGL_RS11610: SGL_RS11610 - class I SAM-dependent methyltransferase, at 2,061,101 to 2,062,621
_RS11610
SGL_RS11615: SGL_RS11615 - ATP-dependent chaperone ClpB, at 2,062,905 to 2,065,523
_RS11615
SGL_RS11620: SGL_RS11620 - hypothetical protein, at 2,065,509 to 2,065,808
_RS11620
SGL_RS11625: SGL_RS11625 - phosphoribulokinase, at 2,066,001 to 2,066,999
_RS11625
Position (kb)
2062
2063
2064
2065
2066 Strain fitness (log2 ratio)
-1
0
1
2 at 2062.093 kb on - strand, within SGL_RS11610 at 2062.553 kb on - strand at 2062.802 kb on - strand at 2063.073 kb on - strand at 2063.099 kb on + strand at 2063.100 kb on + strand at 2063.141 kb on + strand at 2063.347 kb on + strand, within SGL_RS11615 at 2063.355 kb on - strand, within SGL_RS11615 at 2063.515 kb on - strand, within SGL_RS11615 at 2064.680 kb on + strand, within SGL_RS11615 at 2064.747 kb on - strand, within SGL_RS11615 at 2065.014 kb on + strand, within SGL_RS11615 at 2065.169 kb on - strand, within SGL_RS11615 at 2065.321 kb on - strand at 2065.582 kb on - strand, within SGL_RS11620 at 2065.582 kb on - strand, within SGL_RS11620 at 2065.615 kb on - strand, within SGL_RS11620 at 2065.939 kb on - strand at 2065.998 kb on + strand at 2065.998 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 2,062,093 - SGL_RS11610 0.65 -0.1 2,062,553 - -1.1 2,062,802 - -0.4 2,063,073 - +0.3 2,063,099 + +2.2 2,063,100 + +0.1 2,063,141 + +0.5 2,063,347 + SGL_RS11615 0.17 -0.2 2,063,355 - SGL_RS11615 0.17 +0.0 2,063,515 - SGL_RS11615 0.23 -0.2 2,064,680 + SGL_RS11615 0.68 +0.2 2,064,747 - SGL_RS11615 0.70 +1.9 2,065,014 + SGL_RS11615 0.81 -0.0 2,065,169 - SGL_RS11615 0.86 +0.4 2,065,321 - +0.3 2,065,582 - SGL_RS11620 0.24 -0.2 2,065,582 - SGL_RS11620 0.24 +0.3 2,065,615 - SGL_RS11620 0.35 -1.1 2,065,939 - -0.1 2,065,998 + +0.2 2,065,998 + +0.4
Or see this region's nucleotide sequence