Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11315 and SGL_RS11320 are separated by 46 nucleotides SGL_RS11320 and SGL_RS11325 overlap by 4 nucleotides SGL_RS11325 and SGL_RS11330 are separated by 160 nucleotides
SGL_RS11315: SGL_RS11315 - Mur ligase family protein, at 2,000,815 to 2,002,194
_RS11315
SGL_RS11320: SGL_RS11320 - precorrin-8X methylmutase, at 2,002,241 to 2,002,894
_RS11320
SGL_RS11325: SGL_RS11325 - pitrilysin family protein, at 2,002,891 to 2,004,465
_RS11325
SGL_RS11330: SGL_RS11330 - 9,9'-di-cis-zeta-carotene desaturase, at 2,004,626 to 2,006,095
_RS11330
Position (kb)
2002
2003
2004
2005 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2002.208 kb on + strand at 2002.209 kb on + strand at 2002.603 kb on - strand, within SGL_RS11320 at 2002.649 kb on + strand, within SGL_RS11320 at 2002.783 kb on + strand, within SGL_RS11320 at 2002.851 kb on - strand at 2003.216 kb on - strand, within SGL_RS11325 at 2003.501 kb on + strand, within SGL_RS11325 at 2003.525 kb on + strand, within SGL_RS11325 at 2003.787 kb on - strand, within SGL_RS11325 at 2003.837 kb on + strand, within SGL_RS11325 at 2003.951 kb on - strand, within SGL_RS11325 at 2003.971 kb on - strand, within SGL_RS11325 at 2003.971 kb on - strand, within SGL_RS11325 at 2004.072 kb on - strand, within SGL_RS11325 at 2004.196 kb on - strand, within SGL_RS11325 at 2004.220 kb on - strand, within SGL_RS11325 at 2004.699 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 2,002,208 + -0.2 2,002,209 + +0.2 2,002,603 - SGL_RS11320 0.55 -2.4 2,002,649 + SGL_RS11320 0.62 -2.9 2,002,783 + SGL_RS11320 0.83 -1.6 2,002,851 - -3.8 2,003,216 - SGL_RS11325 0.21 -2.6 2,003,501 + SGL_RS11325 0.39 -2.3 2,003,525 + SGL_RS11325 0.40 -1.6 2,003,787 - SGL_RS11325 0.57 -0.5 2,003,837 + SGL_RS11325 0.60 -4.5 2,003,951 - SGL_RS11325 0.67 -1.5 2,003,971 - SGL_RS11325 0.69 -0.6 2,003,971 - SGL_RS11325 0.69 -0.3 2,004,072 - SGL_RS11325 0.75 -1.6 2,004,196 - SGL_RS11325 0.83 -0.8 2,004,220 - SGL_RS11325 0.84 +1.2 2,004,699 + -2.4
Or see this region's nucleotide sequence