Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS20030 and SGL_RS10680 are separated by 29 nucleotides SGL_RS10680 and SGL_RS10685 are separated by 184 nucleotides SGL_RS10685 and SGL_RS10690 are separated by 81 nucleotides SGL_RS10690 and SGL_RS10695 are separated by 39 nucleotides
SGL_RS20030: SGL_RS20030 - iron uptake porin, at 1,864,239 to 1,865,003
_RS20030
SGL_RS10680: SGL_RS10680 - hypothetical protein, at 1,865,033 to 1,865,473
_RS10680
SGL_RS10685: SGL_RS10685 - type IV pilus assembly protein PilM, at 1,865,658 to 1,866,761
_RS10685
SGL_RS10690: SGL_RS10690 - PilN domain-containing protein, at 1,866,843 to 1,867,697
_RS10690
SGL_RS10695: SGL_RS10695 - pilus assembly protein, at 1,867,737 to 1,868,564
_RS10695
Position (kb)
1865
1866
1867 Strain fitness (log2 ratio)
-1
0
1 at 1864.987 kb on - strand at 1865.053 kb on - strand at 1865.118 kb on + strand, within SGL_RS10680 at 1865.409 kb on - strand, within SGL_RS10680 at 1865.425 kb on + strand, within SGL_RS10680 at 1865.425 kb on + strand, within SGL_RS10680 at 1865.433 kb on - strand at 1865.521 kb on + strand at 1865.907 kb on + strand, within SGL_RS10685 at 1865.955 kb on + strand, within SGL_RS10685 at 1866.008 kb on + strand, within SGL_RS10685 at 1866.017 kb on - strand, within SGL_RS10685 at 1866.384 kb on + strand, within SGL_RS10685 at 1866.442 kb on - strand, within SGL_RS10685 at 1866.732 kb on + strand at 1866.740 kb on - strand at 1866.740 kb on - strand at 1866.797 kb on - strand at 1866.921 kb on - strand at 1867.009 kb on - strand, within SGL_RS10690 at 1867.068 kb on + strand, within SGL_RS10690 at 1867.218 kb on + strand, within SGL_RS10690 at 1867.481 kb on + strand, within SGL_RS10690 at 1867.660 kb on - strand at 1867.701 kb on + strand at 1867.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 1,864,987 - -0.1 1,865,053 - +0.6 1,865,118 + SGL_RS10680 0.19 +0.0 1,865,409 - SGL_RS10680 0.85 +0.2 1,865,425 + SGL_RS10680 0.89 -0.3 1,865,425 + SGL_RS10680 0.89 -0.1 1,865,433 - +0.1 1,865,521 + +0.4 1,865,907 + SGL_RS10685 0.23 +0.0 1,865,955 + SGL_RS10685 0.27 +1.7 1,866,008 + SGL_RS10685 0.32 +0.1 1,866,017 - SGL_RS10685 0.33 +0.3 1,866,384 + SGL_RS10685 0.66 +0.1 1,866,442 - SGL_RS10685 0.71 +0.5 1,866,732 + +0.2 1,866,740 - +0.1 1,866,740 - +0.6 1,866,797 - +0.2 1,866,921 - +0.4 1,867,009 - SGL_RS10690 0.19 +0.1 1,867,068 + SGL_RS10690 0.26 +0.2 1,867,218 + SGL_RS10690 0.44 +0.1 1,867,481 + SGL_RS10690 0.75 -0.1 1,867,660 - +0.3 1,867,701 + -0.1 1,867,749 + +0.4
Or see this region's nucleotide sequence