Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08735 and SGL_RS18815 are separated by 88 nucleotides SGL_RS18815 and SGL_RS08740 are separated by 82 nucleotides SGL_RS08740 and SGL_RS08745 are separated by 176 nucleotides
SGL_RS08735: SGL_RS08735 - DUF3488 and DUF4129 domain-containing transglutaminase family protein, at 1,464,954 to 1,467,266
_RS08735
SGL_RS18815: SGL_RS18815 - transposase, at 1,467,355 to 1,467,693
_RS18815
SGL_RS08740: SGL_RS08740 - hypothetical protein, at 1,467,776 to 1,467,976
_RS08740
SGL_RS08745: SGL_RS08745 - ATP-dependent sacrificial sulfur transferase LarE, at 1,468,153 to 1,468,959
_RS08745
Position (kb)
1467
1468 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1466.832 kb on - strand, within SGL_RS08735 at 1466.868 kb on + strand, within SGL_RS08735 at 1466.891 kb on + strand, within SGL_RS08735 at 1466.909 kb on - strand, within SGL_RS08735 at 1467.075 kb on - strand at 1467.116 kb on - strand at 1467.116 kb on - strand at 1467.116 kb on - strand at 1467.156 kb on + strand at 1467.235 kb on + strand at 1467.243 kb on - strand at 1467.255 kb on + strand at 1467.650 kb on + strand, within SGL_RS18815 at 1467.798 kb on + strand, within SGL_RS08740 at 1467.841 kb on - strand, within SGL_RS08740 at 1467.841 kb on - strand, within SGL_RS08740 at 1467.851 kb on - strand, within SGL_RS08740 at 1467.965 kb on + strand at 1468.231 kb on + strand at 1468.308 kb on - strand, within SGL_RS08745 at 1468.375 kb on - strand, within SGL_RS08745 at 1468.559 kb on - strand, within SGL_RS08745 at 1468.644 kb on + strand, within SGL_RS08745 at 1468.644 kb on + strand, within SGL_RS08745 at 1468.912 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 1,466,832 - SGL_RS08735 0.81 -0.1 1,466,868 + SGL_RS08735 0.83 +0.1 1,466,891 + SGL_RS08735 0.84 -0.2 1,466,909 - SGL_RS08735 0.85 -0.0 1,467,075 - +0.3 1,467,116 - -0.0 1,467,116 - +0.1 1,467,116 - -0.3 1,467,156 + -0.4 1,467,235 + -0.3 1,467,243 - +0.1 1,467,255 + +0.1 1,467,650 + SGL_RS18815 0.87 -0.3 1,467,798 + SGL_RS08740 0.11 +0.4 1,467,841 - SGL_RS08740 0.32 +0.1 1,467,841 - SGL_RS08740 0.32 +0.6 1,467,851 - SGL_RS08740 0.37 -0.1 1,467,965 + +0.6 1,468,231 + -2.9 1,468,308 - SGL_RS08745 0.19 -3.8 1,468,375 - SGL_RS08745 0.28 -2.7 1,468,559 - SGL_RS08745 0.50 -3.5 1,468,644 + SGL_RS08745 0.61 -3.5 1,468,644 + SGL_RS08745 0.61 -3.1 1,468,912 - -1.7
Or see this region's nucleotide sequence