Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02190 and SGL_RS02195 are separated by 150 nucleotides SGL_RS02195 and SGL_RS02200 are separated by 79 nucleotides SGL_RS02200 and SGL_RS02205 are separated by 86 nucleotides
SGL_RS02190: SGL_RS02190 - A24 family peptidase, at 74,995 to 75,804
_RS02190
SGL_RS02195: SGL_RS02195 - nucleoside monophosphate kinase, at 75,955 to 76,500
_RS02195
SGL_RS02200: SGL_RS02200 - 4-hydroxy-tetrahydrodipicolinate reductase, at 76,580 to 77,407
_RS02200
SGL_RS02205: SGL_RS02205 - thioredoxin family protein, at 77,494 to 77,826
_RS02205
Position (kb) 75
76
77 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 74.960 kb on + strand at 75.037 kb on + strand at 75.149 kb on + strand, within SGL_RS02190 at 75.268 kb on + strand, within SGL_RS02190 at 75.357 kb on - strand, within SGL_RS02190 at 75.375 kb on + strand, within SGL_RS02190 at 75.685 kb on + strand, within SGL_RS02190 at 75.954 kb on + strand at 75.961 kb on - strand at 75.993 kb on - strand at 76.013 kb on - strand, within SGL_RS02195 at 76.172 kb on + strand, within SGL_RS02195 at 76.179 kb on - strand, within SGL_RS02195 at 76.880 kb on - strand, within SGL_RS02200 at 76.880 kb on - strand, within SGL_RS02200 at 77.149 kb on + strand, within SGL_RS02200 at 77.198 kb on - strand, within SGL_RS02200 at 77.205 kb on - strand, within SGL_RS02200 at 77.440 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 74,960 + +0.2 75,037 + -2.6 75,149 + SGL_RS02190 0.19 -4.3 75,268 + SGL_RS02190 0.34 -2.6 75,357 - SGL_RS02190 0.45 -2.3 75,375 + SGL_RS02190 0.47 -2.8 75,685 + SGL_RS02190 0.85 -1.4 75,954 + +0.3 75,961 - +0.1 75,993 - +0.0 76,013 - SGL_RS02195 0.11 -2.6 76,172 + SGL_RS02195 0.40 +0.5 76,179 - SGL_RS02195 0.41 +1.1 76,880 - SGL_RS02200 0.36 -3.7 76,880 - SGL_RS02200 0.36 -2.3 77,149 + SGL_RS02200 0.69 -1.9 77,198 - SGL_RS02200 0.75 -2.8 77,205 - SGL_RS02200 0.75 -2.8 77,440 - -0.0
Or see this region's nucleotide sequence