Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01055 and SGL_RS01060 overlap by 4 nucleotides SGL_RS01060 and SGL_RS01065 are separated by 3 nucleotides
SGL_RS01055: SGL_RS01055 - CRISPR-associated protein Csx18, at 88,262 to 88,630
_RS01055
SGL_RS01060: SGL_RS01060 - CRISPR-associated endonuclease Cas1, at 88,627 to 89,616
_RS01060
SGL_RS01065: SGL_RS01065 - CRISPR-associated endonuclease Cas2, at 89,620 to 89,898
_RS01065
Position (kb)
89
90 Strain fitness (log2 ratio)
-1
0
1 at 88.751 kb on - strand, within SGL_RS01060 at 88.762 kb on + strand, within SGL_RS01060 at 88.767 kb on + strand, within SGL_RS01060 at 88.769 kb on - strand, within SGL_RS01060 at 88.770 kb on - strand, within SGL_RS01060 at 88.897 kb on - strand, within SGL_RS01060 at 88.897 kb on - strand, within SGL_RS01060 at 88.910 kb on + strand, within SGL_RS01060 at 89.038 kb on - strand, within SGL_RS01060 at 89.040 kb on - strand, within SGL_RS01060 at 89.054 kb on - strand, within SGL_RS01060 at 89.080 kb on + strand, within SGL_RS01060 at 89.259 kb on + strand, within SGL_RS01060 at 89.263 kb on + strand, within SGL_RS01060 at 89.272 kb on - strand, within SGL_RS01060 at 89.341 kb on + strand, within SGL_RS01060 at 89.744 kb on - strand, within SGL_RS01065 at 89.824 kb on + strand, within SGL_RS01065 at 89.825 kb on + strand, within SGL_RS01065 at 89.825 kb on + strand, within SGL_RS01065 at 89.830 kb on + strand, within SGL_RS01065 at 89.866 kb on - strand, within SGL_RS01065 at 89.866 kb on - strand, within SGL_RS01065 at 89.868 kb on + strand, within SGL_RS01065 at 90.099 kb on + strand at 90.148 kb on - strand at 90.318 kb on - strand at 90.579 kb on - strand at 90.727 kb on + strand at 90.735 kb on - strand at 90.735 kb on - strand at 90.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 88,751 - SGL_RS01060 0.13 -0.1 88,762 + SGL_RS01060 0.14 -0.2 88,767 + SGL_RS01060 0.14 +0.6 88,769 - SGL_RS01060 0.14 +0.3 88,770 - SGL_RS01060 0.14 +0.7 88,897 - SGL_RS01060 0.27 +0.1 88,897 - SGL_RS01060 0.27 -0.1 88,910 + SGL_RS01060 0.29 +0.4 89,038 - SGL_RS01060 0.42 +0.1 89,040 - SGL_RS01060 0.42 -0.8 89,054 - SGL_RS01060 0.43 -0.2 89,080 + SGL_RS01060 0.46 +0.1 89,259 + SGL_RS01060 0.64 +0.0 89,263 + SGL_RS01060 0.64 -0.0 89,272 - SGL_RS01060 0.65 +0.1 89,341 + SGL_RS01060 0.72 +0.2 89,744 - SGL_RS01065 0.44 +0.5 89,824 + SGL_RS01065 0.73 +0.7 89,825 + SGL_RS01065 0.73 +0.7 89,825 + SGL_RS01065 0.73 -0.3 89,830 + SGL_RS01065 0.75 -0.1 89,866 - SGL_RS01065 0.88 +0.3 89,866 - SGL_RS01065 0.88 +0.2 89,868 + SGL_RS01065 0.89 +0.2 90,099 + -0.0 90,148 - -0.3 90,318 - -1.5 90,579 - +0.2 90,727 + -0.1 90,735 - +0.1 90,735 - -0.1 90,791 + +0.9
Or see this region's nucleotide sequence