Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00650 and SGL_RS00655 are separated by 75 nucleotides SGL_RS00655 and SGL_RS00660 are separated by 2 nucleotides SGL_RS00660 and SGL_RS00665 are separated by 421 nucleotides
SGL_RS00650: SGL_RS00650 - site-specific integrase, at 2,399 to 2,965
_RS00650
SGL_RS00655: SGL_RS00655 - YlcI/YnfO family protein, at 3,041 to 3,364
_RS00655
SGL_RS00660: SGL_RS00660 - putative toxin-antitoxin system toxin component, PIN family, at 3,367 to 3,819
_RS00660
SGL_RS00665: SGL_RS00665 - IS4-like element IS4Sa family transposase, at 4,241 to 5,257
_RS00665
Position (kb) 3
4 Strain fitness (log2 ratio) -1
0
1 at 2.057 kb on + strand at 2.444 kb on + strand at 2.458 kb on + strand, within SGL_RS00650 at 2.548 kb on + strand, within SGL_RS00650 at 2.551 kb on - strand, within SGL_RS00650 at 3.263 kb on + strand, within SGL_RS00655 at 3.562 kb on - strand, within SGL_RS00660 at 3.648 kb on + strand, within SGL_RS00660 at 3.817 kb on + strand at 3.837 kb on + strand at 3.838 kb on - strand at 3.878 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 2,057 + +0.2 2,444 + -0.2 2,458 + SGL_RS00650 0.10 +0.1 2,548 + SGL_RS00650 0.26 -1.5 2,551 - SGL_RS00650 0.27 +0.2 3,263 + SGL_RS00655 0.69 +0.4 3,562 - SGL_RS00660 0.43 -0.0 3,648 + SGL_RS00660 0.62 -0.0 3,817 + -0.2 3,837 + +0.1 3,838 - +0.3 3,878 + +0.4
Or see this region's nucleotide sequence