Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00530 and SGL_RS00535 are separated by 478 nucleotides SGL_RS00535 and SGL_RS00540 are separated by 43 nucleotides SGL_RS00540 and SGL_RS00545 are separated by 165 nucleotides
SGL_RS00530: SGL_RS00530 - hypothetical protein, at 104,141 to 105,274
_RS00530
SGL_RS00535: SGL_RS00535 - hypothetical protein, at 105,753 to 106,043
_RS00535
SGL_RS00540: SGL_RS00540 - hypothetical protein, at 106,087 to 106,371
_RS00540
SGL_RS00545: SGL_RS00545 - alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB, at 106,537 to 106,950
_RS00545
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-1
0
1 at 105.162 kb on + strand at 105.246 kb on - strand at 105.310 kb on - strand at 105.318 kb on + strand at 105.318 kb on + strand at 105.488 kb on - strand at 105.541 kb on + strand at 105.616 kb on - strand at 105.620 kb on + strand at 105.630 kb on - strand at 105.712 kb on - strand at 105.982 kb on + strand, within SGL_RS00535 at 106.036 kb on - strand at 106.045 kb on + strand at 106.053 kb on - strand at 106.178 kb on + strand, within SGL_RS00540 at 106.190 kb on + strand, within SGL_RS00540 at 106.242 kb on + strand, within SGL_RS00540 at 106.348 kb on - strand at 106.682 kb on - strand, within SGL_RS00545 at 106.795 kb on + strand, within SGL_RS00545 at 106.848 kb on + strand, within SGL_RS00545 at 106.913 kb on + strand at 106.915 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 105,162 + -0.0 105,246 - +0.5 105,310 - +0.1 105,318 + +1.0 105,318 + +0.4 105,488 - +0.1 105,541 + +0.4 105,616 - -0.1 105,620 + +0.4 105,630 - -0.0 105,712 - +0.1 105,982 + SGL_RS00535 0.79 +0.2 106,036 - -0.2 106,045 + -0.7 106,053 - +0.0 106,178 + SGL_RS00540 0.32 +0.5 106,190 + SGL_RS00540 0.36 +0.3 106,242 + SGL_RS00540 0.54 +0.7 106,348 - -0.2 106,682 - SGL_RS00545 0.35 +0.2 106,795 + SGL_RS00545 0.62 +0.1 106,848 + SGL_RS00545 0.75 -0.0 106,913 + -0.2 106,915 + +0.2
Or see this region's nucleotide sequence