Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17705 and SGL_RS17710 overlap by 8 nucleotides SGL_RS17710 and SGL_RS17715 are separated by 552 nucleotides SGL_RS17715 and SGL_RS17720 are separated by 369 nucleotides SGL_RS17720 and SGL_RS17725 are separated by 21 nucleotides
SGL_RS17705: SGL_RS17705 - type II toxin-antitoxin system HicA family toxin, at 3,426,774 to 3,427,001
_RS17705
SGL_RS17710: SGL_RS17710 - type II toxin-antitoxin system HicB family antitoxin, at 3,426,994 to 3,427,203
_RS17710
SGL_RS17715: SGL_RS17715 - DUF4327 family protein, at 3,427,756 to 3,428,013
_RS17715
SGL_RS17720: SGL_RS17720 - NYN domain-containing protein, at 3,428,383 to 3,428,940
_RS17720
SGL_RS17725: SGL_RS17725 - type II toxin-antitoxin system HicB family antitoxin, at 3,428,962 to 3,429,171
_RS17725
Position (kb)
3427
3428
3429 Strain fitness (log2 ratio)
-2
-1
0
1 at 3427.006 kb on - strand at 3427.278 kb on + strand at 3427.278 kb on + strand at 3427.302 kb on + strand at 3427.310 kb on - strand at 3427.343 kb on + strand at 3427.367 kb on + strand at 3427.430 kb on - strand at 3427.444 kb on + strand at 3427.686 kb on + strand at 3427.705 kb on + strand at 3427.742 kb on - strand at 3427.742 kb on - strand at 3427.751 kb on + strand at 3427.858 kb on + strand, within SGL_RS17715 at 3427.865 kb on + strand, within SGL_RS17715 at 3428.061 kb on + strand at 3428.061 kb on + strand at 3428.061 kb on + strand at 3428.075 kb on + strand at 3428.088 kb on - strand at 3428.088 kb on - strand at 3428.133 kb on + strand at 3428.151 kb on + strand at 3428.182 kb on + strand at 3428.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 3,427,006 - +0.2 3,427,278 + +0.1 3,427,278 + -0.1 3,427,302 + +0.6 3,427,310 - +0.0 3,427,343 + +0.1 3,427,367 + -0.4 3,427,430 - -2.0 3,427,444 + +0.1 3,427,686 + +0.3 3,427,705 + +0.1 3,427,742 - +0.2 3,427,742 - +0.0 3,427,751 + +0.1 3,427,858 + SGL_RS17715 0.40 -1.5 3,427,865 + SGL_RS17715 0.42 -1.0 3,428,061 + +0.1 3,428,061 + +0.7 3,428,061 + -0.3 3,428,075 + -0.0 3,428,088 - +0.2 3,428,088 - +0.2 3,428,133 + +0.1 3,428,151 + +0.1 3,428,182 + +0.2 3,428,894 - -2.0
Or see this region's nucleotide sequence