Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17260 and SGL_RS17265 are separated by 72 nucleotides SGL_RS17265 and SGL_RS17270 are separated by 200 nucleotides SGL_RS17270 and SGL_RS17275 are separated by 2 nucleotides SGL_RS17275 and SGL_RS17280 are separated by 37 nucleotides
SGL_RS17260: SGL_RS17260 - 23S ribosomal RNA, at 3,327,007 to 3,329,890
_RS17260
SGL_RS17265: SGL_RS17265 - 5S ribosomal RNA, at 3,329,963 to 3,330,080
_RS17265
SGL_RS17270: SGL_RS17270 - hypothetical protein, at 3,330,281 to 3,331,042
_RS17270
SGL_RS17275: SGL_RS17275 - DUF427 domain-containing protein, at 3,331,045 to 3,331,587
_RS17275
SGL_RS17280: SGL_RS17280 - GNAT family N-acetyltransferase, at 3,331,625 to 3,332,821
_RS17280
Position (kb)
3330
3331
3332 Strain fitness (log2 ratio)
-2
-1
0
1 at 3330.930 kb on + strand, within SGL_RS17270 at 3330.950 kb on + strand, within SGL_RS17270 at 3330.960 kb on - strand, within SGL_RS17270 at 3331.043 kb on - strand at 3331.347 kb on + strand, within SGL_RS17275 at 3331.355 kb on - strand, within SGL_RS17275 at 3331.633 kb on + strand at 3331.702 kb on - strand at 3331.763 kb on + strand, within SGL_RS17280 at 3331.785 kb on + strand, within SGL_RS17280 at 3331.820 kb on - strand, within SGL_RS17280 at 3331.822 kb on - strand, within SGL_RS17280 at 3331.822 kb on - strand, within SGL_RS17280 at 3331.931 kb on - strand, within SGL_RS17280
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 3,330,930 + SGL_RS17270 0.85 -1.4 3,330,950 + SGL_RS17270 0.88 -2.0 3,330,960 - SGL_RS17270 0.89 -1.3 3,331,043 - -0.7 3,331,347 + SGL_RS17275 0.56 -0.3 3,331,355 - SGL_RS17275 0.57 -0.0 3,331,633 + +0.0 3,331,702 - +0.3 3,331,763 + SGL_RS17280 0.12 -0.2 3,331,785 + SGL_RS17280 0.13 -0.3 3,331,820 - SGL_RS17280 0.16 -0.2 3,331,822 - SGL_RS17280 0.16 -0.1 3,331,822 - SGL_RS17280 0.16 +0.1 3,331,931 - SGL_RS17280 0.26 +0.1
Or see this region's nucleotide sequence