Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15645 and SGL_RS15650 are separated by 363 nucleotides SGL_RS15650 and SGL_RS15655 are separated by 175 nucleotides SGL_RS15655 and SGL_RS15660 are separated by 58 nucleotides SGL_RS15660 and SGL_RS15665 are separated by 169 nucleotides
SGL_RS15645: SGL_RS15645 - NAD(P)/FAD-dependent oxidoreductase, at 2,959,530 to 2,960,756
_RS15645
SGL_RS15650: SGL_RS15650 - thioredoxin, at 2,961,120 to 2,961,443
_RS15650
SGL_RS15655: SGL_RS15655 - asparaginase, at 2,961,619 to 2,962,647
_RS15655
SGL_RS15660: SGL_RS15660 - CGLD27 family protein, at 2,962,706 to 2,963,227
_RS15660
SGL_RS15665: SGL_RS15665 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 2,963,397 to 2,964,512
_RS15665
Position (kb)
2961
2962
2963 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2960.630 kb on + strand, within SGL_RS15645 at 2960.768 kb on + strand at 2960.768 kb on + strand at 2960.768 kb on + strand at 2960.884 kb on - strand at 2961.376 kb on - strand, within SGL_RS15650 at 2961.481 kb on + strand at 2961.617 kb on + strand at 2961.735 kb on + strand, within SGL_RS15655 at 2961.742 kb on + strand, within SGL_RS15655 at 2961.793 kb on + strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.801 kb on - strand, within SGL_RS15655 at 2961.811 kb on - strand, within SGL_RS15655 at 2961.926 kb on + strand, within SGL_RS15655 at 2961.934 kb on - strand, within SGL_RS15655 at 2962.013 kb on + strand, within SGL_RS15655 at 2962.053 kb on + strand, within SGL_RS15655 at 2962.112 kb on + strand, within SGL_RS15655 at 2962.152 kb on + strand, within SGL_RS15655 at 2962.311 kb on + strand, within SGL_RS15655 at 2962.316 kb on + strand, within SGL_RS15655 at 2962.338 kb on - strand, within SGL_RS15655 at 2962.417 kb on + strand, within SGL_RS15655 at 2962.449 kb on + strand, within SGL_RS15655 at 2962.495 kb on + strand, within SGL_RS15655 at 2962.549 kb on - strand at 2962.707 kb on - strand at 2962.768 kb on - strand, within SGL_RS15660 at 2962.921 kb on + strand, within SGL_RS15660 at 2962.934 kb on - strand, within SGL_RS15660 at 2963.075 kb on + strand, within SGL_RS15660 at 2963.075 kb on + strand, within SGL_RS15660 at 2963.082 kb on - strand, within SGL_RS15660 at 2963.089 kb on + strand, within SGL_RS15660 at 2963.118 kb on - strand, within SGL_RS15660 at 2963.190 kb on + strand at 2963.198 kb on - strand at 2963.203 kb on - strand at 2963.257 kb on + strand at 2963.303 kb on - strand at 2963.369 kb on + strand at 2963.393 kb on + strand at 2963.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 2,960,630 + SGL_RS15645 0.90 -0.1 2,960,768 + +2.8 2,960,768 + -0.8 2,960,768 + +0.1 2,960,884 - -0.0 2,961,376 - SGL_RS15650 0.79 -1.7 2,961,481 + +0.1 2,961,617 + -0.1 2,961,735 + SGL_RS15655 0.11 -4.0 2,961,742 + SGL_RS15655 0.12 -3.3 2,961,793 + SGL_RS15655 0.17 -3.3 2,961,801 - SGL_RS15655 0.18 -3.9 2,961,801 - SGL_RS15655 0.18 -1.3 2,961,801 - SGL_RS15655 0.18 -5.5 2,961,801 - SGL_RS15655 0.18 -5.1 2,961,811 - SGL_RS15655 0.19 -5.2 2,961,926 + SGL_RS15655 0.30 -2.9 2,961,934 - SGL_RS15655 0.31 -3.6 2,962,013 + SGL_RS15655 0.38 -4.8 2,962,053 + SGL_RS15655 0.42 -4.6 2,962,112 + SGL_RS15655 0.48 -3.7 2,962,152 + SGL_RS15655 0.52 -4.0 2,962,311 + SGL_RS15655 0.67 -3.1 2,962,316 + SGL_RS15655 0.68 -3.4 2,962,338 - SGL_RS15655 0.70 -4.0 2,962,417 + SGL_RS15655 0.78 -4.2 2,962,449 + SGL_RS15655 0.81 -4.2 2,962,495 + SGL_RS15655 0.85 -3.9 2,962,549 - +0.2 2,962,707 - -0.2 2,962,768 - SGL_RS15660 0.12 +0.2 2,962,921 + SGL_RS15660 0.41 +0.3 2,962,934 - SGL_RS15660 0.44 -0.5 2,963,075 + SGL_RS15660 0.71 -0.7 2,963,075 + SGL_RS15660 0.71 +0.2 2,963,082 - SGL_RS15660 0.72 +0.1 2,963,089 + SGL_RS15660 0.73 +0.2 2,963,118 - SGL_RS15660 0.79 -0.2 2,963,190 + +0.5 2,963,198 - -0.3 2,963,203 - +0.3 2,963,257 + -0.1 2,963,303 - +0.2 2,963,369 + +0.5 2,963,393 + -0.4 2,963,401 - -0.3
Or see this region's nucleotide sequence