Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10125 and SGL_RS10130 are separated by 214 nucleotides SGL_RS10130 and SGL_RS10135 are separated by 156 nucleotides
SGL_RS10125: SGL_RS10125 - glucose-6-phosphate isomerase, at 1,744,498 to 1,746,093
_RS10125
SGL_RS10130: SGL_RS10130 - fatty acid desaturase, at 1,746,308 to 1,747,363
_RS10130
SGL_RS10135: SGL_RS10135 - hypothetical protein, at 1,747,520 to 1,748,893
_RS10135
Position (kb)
1746
1747
1748 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1745.514 kb on + strand, within SGL_RS10125 at 1746.300 kb on - strand at 1746.396 kb on + strand at 1746.452 kb on - strand, within SGL_RS10130 at 1746.462 kb on + strand, within SGL_RS10130 at 1746.506 kb on + strand, within SGL_RS10130 at 1746.548 kb on + strand, within SGL_RS10130 at 1746.581 kb on - strand, within SGL_RS10130 at 1746.652 kb on + strand, within SGL_RS10130 at 1746.652 kb on + strand, within SGL_RS10130 at 1746.806 kb on + strand, within SGL_RS10130 at 1746.840 kb on + strand, within SGL_RS10130 at 1746.840 kb on + strand, within SGL_RS10130 at 1746.853 kb on - strand, within SGL_RS10130 at 1746.931 kb on + strand, within SGL_RS10130 at 1746.961 kb on + strand, within SGL_RS10130 at 1746.962 kb on - strand, within SGL_RS10130 at 1747.031 kb on + strand, within SGL_RS10130 at 1747.107 kb on - strand, within SGL_RS10130 at 1747.142 kb on - strand, within SGL_RS10130 at 1747.308 kb on + strand at 1747.655 kb on + strand at 1747.825 kb on + strand, within SGL_RS10135 at 1748.051 kb on - strand, within SGL_RS10135 at 1748.135 kb on - strand, within SGL_RS10135 at 1748.156 kb on - strand, within SGL_RS10135 at 1748.311 kb on + strand, within SGL_RS10135 at 1748.311 kb on + strand, within SGL_RS10135 at 1748.316 kb on + strand, within SGL_RS10135 at 1748.326 kb on + strand, within SGL_RS10135 at 1748.334 kb on - strand, within SGL_RS10135 at 1748.334 kb on - strand, within SGL_RS10135
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 1,745,514 + SGL_RS10125 0.64 -2.2 1,746,300 - -1.7 1,746,396 + -1.7 1,746,452 - SGL_RS10130 0.14 -1.5 1,746,462 + SGL_RS10130 0.15 -1.4 1,746,506 + SGL_RS10130 0.19 -2.2 1,746,548 + SGL_RS10130 0.23 -0.9 1,746,581 - SGL_RS10130 0.26 -1.3 1,746,652 + SGL_RS10130 0.33 -1.7 1,746,652 + SGL_RS10130 0.33 -1.7 1,746,806 + SGL_RS10130 0.47 -2.2 1,746,840 + SGL_RS10130 0.50 -2.8 1,746,840 + SGL_RS10130 0.50 -2.0 1,746,853 - SGL_RS10130 0.52 -1.8 1,746,931 + SGL_RS10130 0.59 -2.7 1,746,961 + SGL_RS10130 0.62 -0.9 1,746,962 - SGL_RS10130 0.62 -2.1 1,747,031 + SGL_RS10130 0.68 -1.7 1,747,107 - SGL_RS10130 0.76 -2.1 1,747,142 - SGL_RS10130 0.79 -2.1 1,747,308 + -4.3 1,747,655 + +0.1 1,747,825 + SGL_RS10135 0.22 +0.2 1,748,051 - SGL_RS10135 0.39 +0.3 1,748,135 - SGL_RS10135 0.45 +1.0 1,748,156 - SGL_RS10135 0.46 -0.0 1,748,311 + SGL_RS10135 0.58 -0.0 1,748,311 + SGL_RS10135 0.58 -0.6 1,748,316 + SGL_RS10135 0.58 -0.1 1,748,326 + SGL_RS10135 0.59 +0.3 1,748,334 - SGL_RS10135 0.59 +0.2 1,748,334 - SGL_RS10135 0.59 +0.4
Or see this region's nucleotide sequence