Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05225 and SGL_RS05230 are separated by 231 nucleotides SGL_RS05230 and SGL_RS05235 are separated by 146 nucleotides SGL_RS05235 and SGL_RS05240 are separated by 45 nucleotides SGL_RS05240 and SGL_RS05245 are separated by 114 nucleotides
SGL_RS05225: SGL_RS05225 - serine--tRNA ligase, at 722,570 to 723,862
_RS05225
SGL_RS05230: SGL_RS05230 - phycobilisome linker polypeptide, at 724,094 to 724,345
_RS05230
SGL_RS05235: SGL_RS05235 - phycobilisome linker polypeptide, at 724,492 to 725,367
_RS05235
SGL_RS05240: SGL_RS05240 - phycobilisome linker polypeptide, at 725,413 to 726,234
_RS05240
SGL_RS05245: SGL_RS05245 - phycocyanin subunit alpha, at 726,349 to 726,837
_RS05245
Position (kb)
724
725
726 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 724.531 kb on - strand at 724.563 kb on - strand at 724.713 kb on - strand, within SGL_RS05235 at 724.799 kb on + strand, within SGL_RS05235 at 724.950 kb on - strand, within SGL_RS05235 at 725.233 kb on + strand, within SGL_RS05235 at 725.554 kb on - strand, within SGL_RS05240 at 726.100 kb on + strand, within SGL_RS05240 at 726.190 kb on - strand at 726.200 kb on - strand at 726.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 724,531 - -2.1 724,563 - -1.6 724,713 - SGL_RS05235 0.25 -2.1 724,799 + SGL_RS05235 0.35 -1.0 724,950 - SGL_RS05235 0.52 -1.6 725,233 + SGL_RS05235 0.85 -2.7 725,554 - SGL_RS05240 0.17 -1.3 726,100 + SGL_RS05240 0.84 -0.8 726,190 - -3.2 726,200 - -1.6 726,321 + -2.5
Or see this region's nucleotide sequence