Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04225 and SGL_RS19430 are separated by 61 nucleotides SGL_RS19430 and SGL_RS04230 are separated by 2 nucleotides SGL_RS04230 and SGL_RS04235 are separated by 81 nucleotides SGL_RS04235 and SGL_RS04240 are separated by 75 nucleotides
SGL_RS04225: SGL_RS04225 - sensor domain-containing diguanylate cyclase, at 496,450 to 497,844
_RS04225
SGL_RS19430: SGL_RS19430 - hypothetical protein, at 497,906 to 498,073
_RS19430
SGL_RS04230: SGL_RS04230 - exonuclease subunit SbcC, at 498,076 to 501,096
_RS04230
SGL_RS04235: SGL_RS04235 - pentapeptide repeat-containing protein, at 501,178 to 501,966
_RS04235
SGL_RS04240: SGL_RS04240 - 50S ribosomal protein L32, at 502,042 to 502,215
_RS04240
Position (kb) 498
499
500
501
502 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 497.144 kb on + strand, within SGL_RS04225 at 497.612 kb on + strand, within SGL_RS04225 at 497.640 kb on + strand, within SGL_RS04225 at 497.650 kb on - strand, within SGL_RS04225 at 498.031 kb on - strand, within SGL_RS19430 at 498.154 kb on + strand at 498.168 kb on + strand at 498.168 kb on + strand at 498.377 kb on + strand at 498.410 kb on + strand, within SGL_RS04230 at 499.092 kb on + strand, within SGL_RS04230 at 499.284 kb on + strand, within SGL_RS04230 at 499.284 kb on + strand, within SGL_RS04230 at 499.296 kb on + strand, within SGL_RS04230 at 499.975 kb on - strand, within SGL_RS04230 at 500.789 kb on + strand, within SGL_RS04230 at 500.890 kb on + strand at 500.890 kb on + strand at 500.898 kb on - strand at 500.989 kb on + strand at 501.194 kb on - strand at 501.297 kb on - strand, within SGL_RS04235 at 501.660 kb on + strand, within SGL_RS04235 at 501.674 kb on + strand, within SGL_RS04235 at 501.676 kb on - strand, within SGL_RS04235 at 501.910 kb on - strand at 501.920 kb on - strand at 501.932 kb on - strand at 501.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 497,144 + SGL_RS04225 0.50 -0.8 497,612 + SGL_RS04225 0.83 +0.1 497,640 + SGL_RS04225 0.85 +0.0 497,650 - SGL_RS04225 0.86 +0.1 498,031 - SGL_RS19430 0.74 -1.8 498,154 + -0.7 498,168 + -3.1 498,168 + -3.4 498,377 + -1.9 498,410 + SGL_RS04230 0.11 -2.5 499,092 + SGL_RS04230 0.34 -1.8 499,284 + SGL_RS04230 0.40 -4.2 499,284 + SGL_RS04230 0.40 -3.3 499,296 + SGL_RS04230 0.40 -1.3 499,975 - SGL_RS04230 0.63 -4.1 500,789 + SGL_RS04230 0.90 -2.5 500,890 + -2.8 500,890 + -1.3 500,898 - -3.5 500,989 + -3.2 501,194 - -0.0 501,297 - SGL_RS04235 0.15 -0.2 501,660 + SGL_RS04235 0.61 -0.0 501,674 + SGL_RS04235 0.63 +0.2 501,676 - SGL_RS04235 0.63 +0.2 501,910 - +0.5 501,920 - -0.2 501,932 - +0.1 501,966 - +0.2
Or see this region's nucleotide sequence