Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03355 and SGL_RS03360 are separated by 89 nucleotides SGL_RS03360 and SGL_RS03365 are separated by 198 nucleotides SGL_RS03365 and SGL_RS03370 are separated by 52 nucleotides SGL_RS03370 and SGL_RS03375 are separated by 129 nucleotides
SGL_RS03355: SGL_RS03355 - hypothetical protein, at 317,984 to 318,571
_RS03355
SGL_RS03360: SGL_RS03360 - 50S ribosomal protein L28, at 318,661 to 318,897
_RS03360
SGL_RS03365: SGL_RS03365 - Ycf66 family protein, at 319,096 to 320,529
_RS03365
SGL_RS03370: SGL_RS03370 - tRNA-Pro, at 320,582 to 320,655
_RS03370
SGL_RS03375: SGL_RS03375 - hypothetical protein, at 320,785 to 321,447
_RS03375
Position (kb)
319
320
321 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 318.428 kb on + strand, within SGL_RS03355 at 318.436 kb on - strand, within SGL_RS03355 at 318.436 kb on - strand, within SGL_RS03355 at 318.438 kb on - strand, within SGL_RS03355 at 318.508 kb on + strand, within SGL_RS03355 at 318.833 kb on - strand, within SGL_RS03360 at 318.849 kb on - strand, within SGL_RS03360 at 318.924 kb on + strand at 319.009 kb on - strand at 319.932 kb on + strand, within SGL_RS03365 at 320.090 kb on - strand, within SGL_RS03365 at 320.147 kb on - strand, within SGL_RS03365 at 320.183 kb on - strand, within SGL_RS03365 at 320.450 kb on - strand at 320.511 kb on + strand at 320.703 kb on + strand at 320.909 kb on + strand, within SGL_RS03375 at 320.928 kb on + strand, within SGL_RS03375 at 321.116 kb on - strand, within SGL_RS03375 at 321.176 kb on + strand, within SGL_RS03375 at 321.176 kb on + strand, within SGL_RS03375 at 321.176 kb on + strand, within SGL_RS03375 at 321.240 kb on - strand, within SGL_RS03375 at 321.332 kb on + strand, within SGL_RS03375 at 321.351 kb on - strand, within SGL_RS03375 at 321.368 kb on + strand, within SGL_RS03375 at 321.387 kb on + strand at 321.388 kb on + strand at 321.505 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 318,428 + SGL_RS03355 0.76 +0.3 318,436 - SGL_RS03355 0.77 -0.1 318,436 - SGL_RS03355 0.77 +0.6 318,438 - SGL_RS03355 0.77 -0.4 318,508 + SGL_RS03355 0.89 -0.3 318,833 - SGL_RS03360 0.73 +0.2 318,849 - SGL_RS03360 0.79 -0.0 318,924 + +0.2 319,009 - -0.1 319,932 + SGL_RS03365 0.58 -3.1 320,090 - SGL_RS03365 0.69 -2.8 320,147 - SGL_RS03365 0.73 -4.6 320,183 - SGL_RS03365 0.76 -3.0 320,450 - -2.4 320,511 + -4.4 320,703 + +0.6 320,909 + SGL_RS03375 0.19 +0.2 320,928 + SGL_RS03375 0.22 +0.3 321,116 - SGL_RS03375 0.50 -0.1 321,176 + SGL_RS03375 0.59 -0.1 321,176 + SGL_RS03375 0.59 +0.1 321,176 + SGL_RS03375 0.59 -0.0 321,240 - SGL_RS03375 0.69 +0.3 321,332 + SGL_RS03375 0.83 +0.2 321,351 - SGL_RS03375 0.85 +0.5 321,368 + SGL_RS03375 0.88 -1.4 321,387 + +0.1 321,388 + +1.0 321,505 + +0.4
Or see this region's nucleotide sequence