Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02205 and SGL_RS02210 are separated by 153 nucleotides SGL_RS02210 and SGL_RS02215 overlap by 41 nucleotides SGL_RS02215 and SGL_RS02220 are separated by 21 nucleotides SGL_RS02220 and SGL_RS02225 are separated by 137 nucleotides SGL_RS02225 and SGL_RS02230 are separated by 5 nucleotides
SGL_RS02205: SGL_RS02205 - thioredoxin family protein, at 77,494 to 77,826
_RS02205
SGL_RS02210: SGL_RS02210 - DUF2442 domain-containing protein, at 77,980 to 78,246
_RS02210
SGL_RS02215: SGL_RS02215 - DUF4160 domain-containing protein, at 78,206 to 78,445
_RS02215
SGL_RS02220: SGL_RS02220 - phosphoribosylformylglycinamidine synthase subunit PurL, at 78,467 to 80,800
_RS02220
SGL_RS02225: SGL_RS02225 - hypothetical protein, at 80,938 to 81,690
_RS02225
SGL_RS02230: SGL_RS02230 - NUDIX hydrolase, at 81,696 to 82,259
_RS02230
Position (kb) 78
79
80
81 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 77.505 kb on - strand at 77.523 kb on + strand at 77.624 kb on + strand, within SGL_RS02205 at 77.657 kb on + strand, within SGL_RS02205 at 77.708 kb on - strand, within SGL_RS02205 at 77.905 kb on - strand at 78.242 kb on - strand at 78.580 kb on - strand at 78.585 kb on - strand at 78.609 kb on + strand at 78.806 kb on + strand, within SGL_RS02220 at 78.806 kb on + strand, within SGL_RS02220 at 78.840 kb on - strand, within SGL_RS02220 at 79.137 kb on + strand, within SGL_RS02220 at 79.147 kb on + strand, within SGL_RS02220 at 79.380 kb on + strand, within SGL_RS02220 at 79.526 kb on - strand, within SGL_RS02220 at 79.667 kb on + strand, within SGL_RS02220 at 79.958 kb on + strand, within SGL_RS02220 at 80.094 kb on + strand, within SGL_RS02220 at 80.221 kb on + strand, within SGL_RS02220 at 80.288 kb on - strand, within SGL_RS02220 at 81.516 kb on - strand, within SGL_RS02225 at 81.654 kb on + strand at 81.777 kb on - strand, within SGL_RS02230
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 77,505 - -1.1 77,523 + +0.8 77,624 + SGL_RS02205 0.39 +0.0 77,657 + SGL_RS02205 0.49 +0.0 77,708 - SGL_RS02205 0.64 +1.5 77,905 - +0.1 78,242 - -0.0 78,580 - -3.8 78,585 - -2.7 78,609 + -2.1 78,806 + SGL_RS02220 0.15 -2.3 78,806 + SGL_RS02220 0.15 -3.8 78,840 - SGL_RS02220 0.16 -2.5 79,137 + SGL_RS02220 0.29 -3.5 79,147 + SGL_RS02220 0.29 -3.1 79,380 + SGL_RS02220 0.39 -2.9 79,526 - SGL_RS02220 0.45 -2.5 79,667 + SGL_RS02220 0.51 +2.9 79,958 + SGL_RS02220 0.64 -0.1 80,094 + SGL_RS02220 0.70 -3.0 80,221 + SGL_RS02220 0.75 -2.7 80,288 - SGL_RS02220 0.78 -3.9 81,516 - SGL_RS02225 0.77 -2.9 81,654 + -0.5 81,777 - SGL_RS02230 0.14 +0.1
Or see this region's nucleotide sequence