Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01135 and SGL_RS01140 are separated by 38 nucleotides SGL_RS01140 and SGL_RS01145 overlap by 4 nucleotides SGL_RS01145 and SGL_RS01150 overlap by 8 nucleotides SGL_RS01150 and SGL_RS01155 overlap by 4 nucleotides
SGL_RS01135: SGL_RS01135 - DUF4433 domain-containing protein, at 2,983 to 3,621
_RS01135
SGL_RS01140: SGL_RS01140 - hypothetical protein, at 3,660 to 3,839
_RS01140
SGL_RS01145: SGL_RS01145 - zeta toxin family protein, at 3,836 to 4,372
_RS01145
SGL_RS01150: SGL_RS01150 - nucleotidyltransferase family protein, at 4,365 to 4,655
_RS01150
SGL_RS01155: SGL_RS01155 - restriction endonuclease subunit S, at 4,652 to 6,046
_RS01155
Position (kb) 4
5 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3.385 kb on + strand, within SGL_RS01135 at 3.417 kb on - strand, within SGL_RS01135 at 3.459 kb on - strand, within SGL_RS01135 at 3.516 kb on + strand, within SGL_RS01135 at 3.563 kb on - strand at 3.596 kb on + strand at 3.596 kb on + strand at 3.596 kb on + strand at 3.596 kb on + strand at 3.596 kb on + strand at 3.604 kb on - strand at 3.604 kb on - strand at 3.604 kb on - strand at 3.604 kb on - strand at 3.641 kb on + strand at 3.862 kb on + strand at 3.884 kb on + strand at 4.015 kb on + strand, within SGL_RS01145 at 4.035 kb on - strand, within SGL_RS01145 at 4.070 kb on + strand, within SGL_RS01145 at 4.114 kb on + strand, within SGL_RS01145 at 4.132 kb on + strand, within SGL_RS01145 at 4.139 kb on + strand, within SGL_RS01145 at 4.140 kb on - strand, within SGL_RS01145 at 4.161 kb on - strand, within SGL_RS01145 at 4.266 kb on - strand, within SGL_RS01145 at 4.312 kb on - strand, within SGL_RS01145 at 4.534 kb on + strand, within SGL_RS01150 at 4.572 kb on - strand, within SGL_RS01150 at 4.624 kb on - strand, within SGL_RS01150 at 4.650 kb on - strand at 4.659 kb on + strand at 5.140 kb on - strand, within SGL_RS01155 at 5.365 kb on + strand, within SGL_RS01155 at 5.611 kb on + strand, within SGL_RS01155 at 5.611 kb on + strand, within SGL_RS01155 at 5.612 kb on + strand, within SGL_RS01155 at 5.652 kb on - strand, within SGL_RS01155
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 3,385 + SGL_RS01135 0.63 +0.1 3,417 - SGL_RS01135 0.68 -0.2 3,459 - SGL_RS01135 0.74 +0.1 3,516 + SGL_RS01135 0.83 -1.8 3,563 - +0.4 3,596 + -0.7 3,596 + +0.7 3,596 + +1.6 3,596 + +0.2 3,596 + +1.0 3,604 - +0.4 3,604 - +0.1 3,604 - +0.0 3,604 - -0.4 3,641 + +0.4 3,862 + -1.2 3,884 + +0.3 4,015 + SGL_RS01145 0.33 -0.6 4,035 - SGL_RS01145 0.37 +0.2 4,070 + SGL_RS01145 0.44 +0.5 4,114 + SGL_RS01145 0.52 +0.1 4,132 + SGL_RS01145 0.55 -0.1 4,139 + SGL_RS01145 0.56 +0.6 4,140 - SGL_RS01145 0.57 -1.0 4,161 - SGL_RS01145 0.61 +0.7 4,266 - SGL_RS01145 0.80 +0.7 4,312 - SGL_RS01145 0.89 +1.9 4,534 + SGL_RS01150 0.58 -1.9 4,572 - SGL_RS01150 0.71 -2.0 4,624 - SGL_RS01150 0.89 -0.6 4,650 - +0.1 4,659 + +0.3 5,140 - SGL_RS01155 0.35 +0.8 5,365 + SGL_RS01155 0.51 -0.4 5,611 + SGL_RS01155 0.69 +0.1 5,611 + SGL_RS01155 0.69 -0.2 5,612 + SGL_RS01155 0.69 -0.2 5,652 - SGL_RS01155 0.72 -0.0
Or see this region's nucleotide sequence