Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14565 and SGL_RS14570 are separated by 36 nucleotides SGL_RS14570 and SGL_RS14575 are separated by 228 nucleotides SGL_RS14575 and SGL_RS14580 are separated by 222 nucleotides SGL_RS14580 and SGL_RS14585 are separated by 55 nucleotides
SGL_RS14565: SGL_RS14565 - SpoIID/LytB domain-containing protein, at 2,722,431 to 2,724,038
_RS14565
SGL_RS14570: SGL_RS14570 - rhodanese-like domain-containing protein, at 2,724,075 to 2,724,419
_RS14570
SGL_RS14575: SGL_RS14575 - RNA-binding protein, at 2,724,648 to 2,725,103
_RS14575
SGL_RS14580: SGL_RS14580 - ribose-5-phosphate isomerase RpiA, at 2,725,326 to 2,726,033
_RS14580
SGL_RS14585: SGL_RS14585 - TolB family protein, at 2,726,089 to 2,726,640
_RS14585
Position (kb)
2724
2725
2726 Strain fitness (log2 ratio)
-2
-1
0
1 at 2723.651 kb on + strand, within SGL_RS14565 at 2723.794 kb on + strand, within SGL_RS14565 at 2723.808 kb on + strand, within SGL_RS14565 at 2723.923 kb on + strand at 2723.931 kb on - strand at 2724.139 kb on - strand, within SGL_RS14570 at 2724.318 kb on + strand, within SGL_RS14570 at 2724.318 kb on + strand, within SGL_RS14570 at 2724.511 kb on + strand at 2724.771 kb on + strand, within SGL_RS14575 at 2725.059 kb on + strand at 2725.129 kb on - strand at 2725.529 kb on - strand, within SGL_RS14580 at 2725.652 kb on - strand, within SGL_RS14580 at 2726.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 2,723,651 + SGL_RS14565 0.76 +0.3 2,723,794 + SGL_RS14565 0.85 -1.4 2,723,808 + SGL_RS14565 0.86 -0.1 2,723,923 + -0.1 2,723,931 - +0.3 2,724,139 - SGL_RS14570 0.19 +0.0 2,724,318 + SGL_RS14570 0.70 +0.0 2,724,318 + SGL_RS14570 0.70 +0.1 2,724,511 + -0.8 2,724,771 + SGL_RS14575 0.27 -0.9 2,725,059 + +0.0 2,725,129 - +0.0 2,725,529 - SGL_RS14580 0.29 -2.5 2,725,652 - SGL_RS14580 0.46 -0.1 2,726,060 - +0.0
Or see this region's nucleotide sequence