Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10295 and SGL_RS10300 are separated by 86 nucleotides SGL_RS10300 and SGL_RS10305 are separated by 182 nucleotides SGL_RS10305 and SGL_RS10310 are separated by 59 nucleotides SGL_RS10310 and SGL_RS10315 are separated by 274 nucleotides
SGL_RS10295: SGL_RS10295 - GAF domain-containing protein, at 1,782,057 to 1,783,355
_RS10295
SGL_RS10300: SGL_RS10300 - hypothetical protein, at 1,783,442 to 1,783,672
_RS10300
SGL_RS10305: SGL_RS10305 - PIN/TRAM domain-containing protein, at 1,783,855 to 1,784,934
_RS10305
SGL_RS10310: SGL_RS10310 - hypothetical protein, at 1,784,994 to 1,785,560
_RS10310
SGL_RS10315: SGL_RS10315 - hypothetical protein, at 1,785,835 to 1,788,357
_RS10315
Position (kb)
1783
1784
1785 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1782.967 kb on - strand, within SGL_RS10295 at 1782.987 kb on + strand, within SGL_RS10295 at 1783.008 kb on - strand, within SGL_RS10295 at 1783.193 kb on + strand, within SGL_RS10295 at 1783.364 kb on + strand at 1783.377 kb on + strand at 1783.574 kb on + strand, within SGL_RS10300 at 1783.910 kb on - strand at 1784.226 kb on + strand, within SGL_RS10305 at 1784.249 kb on + strand, within SGL_RS10305 at 1784.318 kb on + strand, within SGL_RS10305 at 1784.383 kb on + strand, within SGL_RS10305 at 1784.383 kb on + strand, within SGL_RS10305 at 1784.472 kb on + strand, within SGL_RS10305 at 1784.558 kb on - strand, within SGL_RS10305 at 1784.708 kb on - strand, within SGL_RS10305 at 1784.991 kb on + strand at 1784.991 kb on + strand at 1785.162 kb on - strand, within SGL_RS10310 at 1785.291 kb on + strand, within SGL_RS10310 at 1785.319 kb on - strand, within SGL_RS10310 at 1785.356 kb on - strand, within SGL_RS10310 at 1785.671 kb on + strand at 1785.679 kb on - strand at 1785.726 kb on - strand at 1785.763 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 1,782,967 - SGL_RS10295 0.70 +0.5 1,782,987 + SGL_RS10295 0.72 -0.0 1,783,008 - SGL_RS10295 0.73 +0.0 1,783,193 + SGL_RS10295 0.87 +0.5 1,783,364 + +0.3 1,783,377 + -0.1 1,783,574 + SGL_RS10300 0.57 +0.1 1,783,910 - -2.3 1,784,226 + SGL_RS10305 0.34 -2.5 1,784,249 + SGL_RS10305 0.36 -2.8 1,784,318 + SGL_RS10305 0.43 -2.1 1,784,383 + SGL_RS10305 0.49 -2.8 1,784,383 + SGL_RS10305 0.49 -1.8 1,784,472 + SGL_RS10305 0.57 -2.6 1,784,558 - SGL_RS10305 0.65 -3.0 1,784,708 - SGL_RS10305 0.79 -3.0 1,784,991 + +0.1 1,784,991 + -0.1 1,785,162 - SGL_RS10310 0.30 +0.1 1,785,291 + SGL_RS10310 0.52 +0.1 1,785,319 - SGL_RS10310 0.57 -0.1 1,785,356 - SGL_RS10310 0.64 -0.2 1,785,671 + +0.2 1,785,679 - -0.1 1,785,726 - -0.2 1,785,763 + -0.2
Or see this region's nucleotide sequence