Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09295 and SGL_RS09305 are separated by 553 nucleotides SGL_RS09305 and SGL_RS09310 are separated by 118 nucleotides
SGL_RS09295: SGL_RS09295 - alpha/beta hydrolase, at 1,590,496 to 1,592,364
_RS09295
SGL_RS09305: SGL_RS09305 - adenosyl-hopene transferase HpnH, at 1,592,918 to 1,594,000
_RS09305
SGL_RS09310: SGL_RS09310 - glycosyltransferase family 4 protein, at 1,594,119 to 1,595,288
_RS09310
Position (kb)
1592
1593
1594
1595 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1591.943 kb on + strand, within SGL_RS09295 at 1591.977 kb on - strand, within SGL_RS09295 at 1592.067 kb on + strand, within SGL_RS09295 at 1592.106 kb on + strand, within SGL_RS09295 at 1592.120 kb on + strand, within SGL_RS09295 at 1592.159 kb on - strand, within SGL_RS09295 at 1592.702 kb on - strand at 1592.853 kb on - strand at 1592.853 kb on - strand at 1592.911 kb on + strand at 1593.006 kb on + strand at 1593.088 kb on + strand, within SGL_RS09305 at 1593.106 kb on - strand, within SGL_RS09305 at 1593.185 kb on + strand, within SGL_RS09305 at 1593.193 kb on - strand, within SGL_RS09305 at 1593.193 kb on - strand, within SGL_RS09305 at 1593.209 kb on + strand, within SGL_RS09305 at 1593.209 kb on - strand, within SGL_RS09305 at 1593.265 kb on + strand, within SGL_RS09305 at 1593.295 kb on + strand, within SGL_RS09305 at 1593.384 kb on + strand, within SGL_RS09305 at 1593.392 kb on + strand, within SGL_RS09305 at 1593.576 kb on + strand, within SGL_RS09305 at 1593.584 kb on - strand, within SGL_RS09305 at 1593.584 kb on - strand, within SGL_RS09305 at 1593.594 kb on + strand, within SGL_RS09305 at 1593.755 kb on + strand, within SGL_RS09305 at 1593.929 kb on - strand at 1594.069 kb on + strand at 1594.076 kb on - strand at 1594.310 kb on + strand, within SGL_RS09310 at 1594.554 kb on - strand, within SGL_RS09310
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 1,591,943 + SGL_RS09295 0.77 +0.1 1,591,977 - SGL_RS09295 0.79 -0.3 1,592,067 + SGL_RS09295 0.84 -0.0 1,592,106 + SGL_RS09295 0.86 -0.4 1,592,120 + SGL_RS09295 0.87 +0.6 1,592,159 - SGL_RS09295 0.89 -0.1 1,592,702 - -0.1 1,592,853 - +0.4 1,592,853 - +0.2 1,592,911 + +0.1 1,593,006 + -1.1 1,593,088 + SGL_RS09305 0.16 -1.1 1,593,106 - SGL_RS09305 0.17 -1.8 1,593,185 + SGL_RS09305 0.25 -2.1 1,593,193 - SGL_RS09305 0.25 -0.2 1,593,193 - SGL_RS09305 0.25 +0.0 1,593,209 + SGL_RS09305 0.27 -1.1 1,593,209 - SGL_RS09305 0.27 -0.6 1,593,265 + SGL_RS09305 0.32 -2.5 1,593,295 + SGL_RS09305 0.35 -0.3 1,593,384 + SGL_RS09305 0.43 -0.8 1,593,392 + SGL_RS09305 0.44 -1.5 1,593,576 + SGL_RS09305 0.61 -1.4 1,593,584 - SGL_RS09305 0.61 -1.6 1,593,584 - SGL_RS09305 0.61 -0.5 1,593,594 + SGL_RS09305 0.62 -0.4 1,593,755 + SGL_RS09305 0.77 -0.6 1,593,929 - -1.6 1,594,069 + +0.2 1,594,076 - -0.3 1,594,310 + SGL_RS09310 0.16 -4.3 1,594,554 - SGL_RS09310 0.37 -2.8
Or see this region's nucleotide sequence