Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08750 and SGL_RS08755 are separated by 45 nucleotides SGL_RS08755 and SGL_RS08760 are separated by 40 nucleotides SGL_RS08760 and SGL_RS08765 are separated by 42 nucleotides SGL_RS08765 and SGL_RS08770 are separated by 78 nucleotides SGL_RS08770 and SGL_RS08775 are separated by 129 nucleotides
SGL_RS08750: SGL_RS08750 - YdcF family protein, at 1,469,013 to 1,469,801
_RS08750
SGL_RS08755: SGL_RS08755 - 3-hydroxyacyl-ACP dehydratase FabZ, at 1,469,847 to 1,470,347
_RS08755
SGL_RS08760: SGL_RS08760 - hypothetical protein, at 1,470,388 to 1,470,567
_RS08760
SGL_RS08765: SGL_RS08765 - 2-phosphosulfolactate phosphatase family protein, at 1,470,610 to 1,471,335
_RS08765
SGL_RS08770: SGL_RS08770 - nitroreductase family protein, at 1,471,414 to 1,472,019
_RS08770
SGL_RS08775: SGL_RS08775 - aspartate--tRNA ligase, at 1,472,149 to 1,473,948
_RS08775
Position (kb)
1470
1471
1472 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1469.615 kb on - strand, within SGL_RS08750 at 1469.638 kb on - strand, within SGL_RS08750 at 1470.483 kb on + strand, within SGL_RS08760 at 1470.941 kb on + strand, within SGL_RS08765 at 1470.950 kb on - strand, within SGL_RS08765 at 1470.992 kb on - strand, within SGL_RS08765 at 1471.080 kb on + strand, within SGL_RS08765
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 1,469,615 - SGL_RS08750 0.76 +0.8 1,469,638 - SGL_RS08750 0.79 -0.2 1,470,483 + SGL_RS08760 0.53 -0.3 1,470,941 + SGL_RS08765 0.46 -1.8 1,470,950 - SGL_RS08765 0.47 -3.3 1,470,992 - SGL_RS08765 0.53 -2.0 1,471,080 + SGL_RS08765 0.65 -2.1
Or see this region's nucleotide sequence