Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07125 and SGL_RS07130 are separated by 305 nucleotides SGL_RS07130 and SGL_RS07135 are separated by 126 nucleotides SGL_RS07135 and SGL_RS07140 are separated by 16 nucleotides
SGL_RS07125: SGL_RS07125 - translocation/assembly module TamB domain-containing protein, at 1,124,071 to 1,130,007
_RS07125
SGL_RS07130: SGL_RS07130 - AAA-like domain-containing protein, at 1,130,313 to 1,131,683
_RS07130
SGL_RS07135: SGL_RS07135 - large conductance mechanosensitive channel protein MscL, at 1,131,810 to 1,132,247
_RS07135
SGL_RS07140: SGL_RS07140 - Holliday junction branch migration protein RuvA, at 1,132,264 to 1,132,899
_RS07140
Position (kb)
1130
1131
1132 Strain fitness (log2 ratio)
-1
0
1 at 1129.427 kb on + strand at 1129.434 kb on - strand at 1129.435 kb on - strand at 1129.468 kb on + strand at 1129.580 kb on + strand at 1129.589 kb on - strand at 1129.796 kb on + strand at 1129.876 kb on - strand at 1129.887 kb on + strand at 1129.995 kb on + strand at 1130.058 kb on + strand at 1130.061 kb on - strand at 1130.062 kb on - strand at 1130.063 kb on + strand at 1130.692 kb on + strand, within SGL_RS07130 at 1130.816 kb on - strand, within SGL_RS07130 at 1131.024 kb on - strand, within SGL_RS07130 at 1131.130 kb on - strand, within SGL_RS07130 at 1131.222 kb on + strand, within SGL_RS07130 at 1131.246 kb on - strand, within SGL_RS07130 at 1131.301 kb on + strand, within SGL_RS07130 at 1131.398 kb on + strand, within SGL_RS07130 at 1131.406 kb on - strand, within SGL_RS07130 at 1131.434 kb on + strand, within SGL_RS07130 at 1131.611 kb on + strand at 1131.978 kb on + strand, within SGL_RS07135 at 1131.986 kb on - strand, within SGL_RS07135 at 1131.986 kb on - strand, within SGL_RS07135 at 1132.147 kb on + strand, within SGL_RS07135
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 1,129,427 + -1.1 1,129,434 - +0.1 1,129,435 - -0.5 1,129,468 + +0.4 1,129,580 + -0.6 1,129,589 - -0.1 1,129,796 + -0.3 1,129,876 - +0.1 1,129,887 + -0.1 1,129,995 + -0.2 1,130,058 + +0.1 1,130,061 - -0.1 1,130,062 - +0.1 1,130,063 + +0.3 1,130,692 + SGL_RS07130 0.28 -0.8 1,130,816 - SGL_RS07130 0.37 -1.3 1,131,024 - SGL_RS07130 0.52 -0.8 1,131,130 - SGL_RS07130 0.60 -0.8 1,131,222 + SGL_RS07130 0.66 -1.4 1,131,246 - SGL_RS07130 0.68 -0.9 1,131,301 + SGL_RS07130 0.72 -1.0 1,131,398 + SGL_RS07130 0.79 -0.9 1,131,406 - SGL_RS07130 0.80 -0.8 1,131,434 + SGL_RS07130 0.82 -0.7 1,131,611 + -1.2 1,131,978 + SGL_RS07135 0.38 -0.6 1,131,986 - SGL_RS07135 0.40 -0.3 1,131,986 - SGL_RS07135 0.40 -0.4 1,132,147 + SGL_RS07135 0.77 -0.2
Or see this region's nucleotide sequence