Experiment: control for light_bubbling experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04765 and SGL_RS04770 are separated by 520 nucleotides SGL_RS04770 and SGL_RS04775 are separated by 88 nucleotides SGL_RS04775 and SGL_RS04780 are separated by 90 nucleotides
SGL_RS04765: SGL_RS04765 - TM2 domain-containing protein, at 618,311 to 618,694
_RS04765
SGL_RS04770: SGL_RS04770 - DUF1825 family protein, at 619,215 to 619,529
_RS04770
SGL_RS04775: SGL_RS04775 - alpha/beta hydrolase, at 619,618 to 620,469
_RS04775
SGL_RS04780: SGL_RS04780 - alpha/beta fold hydrolase, at 620,560 to 621,468
_RS04780
Position (kb) 619
620
621 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 618.624 kb on + strand, within SGL_RS04765 at 618.695 kb on - strand at 618.802 kb on + strand at 618.835 kb on - strand at 619.128 kb on + strand at 619.206 kb on - strand at 619.414 kb on - strand, within SGL_RS04770 at 619.521 kb on + strand at 619.561 kb on - strand at 619.657 kb on + strand at 619.961 kb on - strand, within SGL_RS04775 at 619.976 kb on - strand, within SGL_RS04775 at 620.115 kb on + strand, within SGL_RS04775 at 620.369 kb on - strand, within SGL_RS04775 at 620.567 kb on + strand at 620.608 kb on + strand at 620.710 kb on - strand, within SGL_RS04780 at 620.775 kb on - strand, within SGL_RS04780 at 620.970 kb on + strand, within SGL_RS04780 at 620.970 kb on + strand, within SGL_RS04780 at 621.010 kb on + strand, within SGL_RS04780 at 621.018 kb on - strand, within SGL_RS04780 at 621.105 kb on + strand, within SGL_RS04780 at 621.105 kb on + strand, within SGL_RS04780 at 621.149 kb on + strand, within SGL_RS04780 at 621.257 kb on - strand, within SGL_RS04780 at 621.263 kb on + strand, within SGL_RS04780 at 621.292 kb on + strand, within SGL_RS04780 at 621.292 kb on - strand, within SGL_RS04780 at 621.354 kb on + strand, within SGL_RS04780 at 621.362 kb on + strand, within SGL_RS04780 at 621.411 kb on - strand at 621.449 kb on + strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.462 kb on + strand at 621.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction control for light_bubbling experiments remove 618,624 + SGL_RS04765 0.82 -1.6 618,695 - -3.5 618,802 + -0.0 618,835 - +0.1 619,128 + -0.2 619,206 - +0.6 619,414 - SGL_RS04770 0.63 -0.6 619,521 + +1.0 619,561 - +0.2 619,657 + -2.6 619,961 - SGL_RS04775 0.40 -2.7 619,976 - SGL_RS04775 0.42 -2.5 620,115 + SGL_RS04775 0.58 -4.0 620,369 - SGL_RS04775 0.88 -2.1 620,567 + +0.9 620,608 + +0.7 620,710 - SGL_RS04780 0.17 -0.3 620,775 - SGL_RS04780 0.24 -0.0 620,970 + SGL_RS04780 0.45 +0.3 620,970 + SGL_RS04780 0.45 +0.1 621,010 + SGL_RS04780 0.50 -0.1 621,018 - SGL_RS04780 0.50 +0.2 621,105 + SGL_RS04780 0.60 -0.3 621,105 + SGL_RS04780 0.60 +0.2 621,149 + SGL_RS04780 0.65 +0.6 621,257 - SGL_RS04780 0.77 -0.0 621,263 + SGL_RS04780 0.77 +0.2 621,292 + SGL_RS04780 0.81 +0.1 621,292 - SGL_RS04780 0.81 +0.1 621,354 + SGL_RS04780 0.87 +0.1 621,362 + SGL_RS04780 0.88 +0.2 621,411 - +0.5 621,449 + -0.1 621,457 - -0.4 621,457 - +0.8 621,457 - -0.2 621,462 + +0.7 621,462 + -0.1
Or see this region's nucleotide sequence