Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14335 and SGL_RS14340 overlap by 8 nucleotides SGL_RS14340 and SGL_RS14345 are separated by 130 nucleotides SGL_RS14345 and SGL_RS14350 are separated by 14 nucleotides
SGL_RS14335: SGL_RS14335 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 2,670,115 to 2,671,902
_RS14335
SGL_RS14340: SGL_RS14340 - protein phosphatase 2C domain-containing protein, at 2,671,895 to 2,673,970
_RS14340
SGL_RS14345: SGL_RS14345 - VWA domain-containing protein, at 2,674,101 to 2,674,721
_RS14345
SGL_RS14350: SGL_RS14350 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase, at 2,674,736 to 2,676,196
_RS14350
Position (kb)
2671
2672
2673
2674 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2671.039 kb on + strand, within SGL_RS14335 at 2671.085 kb on + strand, within SGL_RS14335 at 2671.338 kb on - strand, within SGL_RS14335 at 2671.667 kb on + strand, within SGL_RS14335 at 2671.885 kb on - strand at 2671.885 kb on - strand at 2672.012 kb on - strand at 2672.077 kb on + strand at 2672.212 kb on + strand, within SGL_RS14340 at 2672.256 kb on - strand, within SGL_RS14340 at 2672.492 kb on - strand, within SGL_RS14340 at 2672.515 kb on - strand, within SGL_RS14340 at 2672.532 kb on + strand, within SGL_RS14340 at 2672.540 kb on - strand, within SGL_RS14340 at 2672.652 kb on - strand, within SGL_RS14340 at 2672.662 kb on + strand, within SGL_RS14340 at 2672.670 kb on - strand, within SGL_RS14340 at 2672.671 kb on - strand, within SGL_RS14340 at 2672.892 kb on + strand, within SGL_RS14340 at 2672.942 kb on - strand, within SGL_RS14340 at 2673.061 kb on + strand, within SGL_RS14340 at 2673.072 kb on + strand, within SGL_RS14340 at 2673.092 kb on - strand, within SGL_RS14340 at 2673.190 kb on - strand, within SGL_RS14340 at 2673.216 kb on + strand, within SGL_RS14340 at 2673.221 kb on + strand, within SGL_RS14340 at 2673.335 kb on + strand, within SGL_RS14340 at 2673.335 kb on + strand, within SGL_RS14340 at 2673.487 kb on - strand, within SGL_RS14340 at 2673.495 kb on + strand, within SGL_RS14340 at 2673.542 kb on - strand, within SGL_RS14340 at 2673.753 kb on - strand, within SGL_RS14340 at 2673.788 kb on + strand at 2673.881 kb on + strand at 2673.961 kb on - strand at 2673.967 kb on + strand at 2673.980 kb on - strand at 2673.980 kb on - strand at 2674.103 kb on - strand at 2674.215 kb on + strand, within SGL_RS14345 at 2674.334 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.368 kb on + strand, within SGL_RS14345 at 2674.376 kb on - strand, within SGL_RS14345 at 2674.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,671,039 + SGL_RS14335 0.52 -2.7 2,671,085 + SGL_RS14335 0.54 -1.6 2,671,338 - SGL_RS14335 0.68 -2.7 2,671,667 + SGL_RS14335 0.87 -2.7 2,671,885 - +0.9 2,671,885 - -2.0 2,672,012 - -1.1 2,672,077 + -3.7 2,672,212 + SGL_RS14340 0.15 -2.7 2,672,256 - SGL_RS14340 0.17 -1.0 2,672,492 - SGL_RS14340 0.29 -0.2 2,672,515 - SGL_RS14340 0.30 -0.7 2,672,532 + SGL_RS14340 0.31 -1.9 2,672,540 - SGL_RS14340 0.31 -0.5 2,672,652 - SGL_RS14340 0.36 -0.5 2,672,662 + SGL_RS14340 0.37 -1.2 2,672,670 - SGL_RS14340 0.37 -0.4 2,672,671 - SGL_RS14340 0.37 -1.5 2,672,892 + SGL_RS14340 0.48 -2.5 2,672,942 - SGL_RS14340 0.50 -0.6 2,673,061 + SGL_RS14340 0.56 -2.7 2,673,072 + SGL_RS14340 0.57 +0.0 2,673,092 - SGL_RS14340 0.58 -0.8 2,673,190 - SGL_RS14340 0.62 -0.9 2,673,216 + SGL_RS14340 0.64 -0.1 2,673,221 + SGL_RS14340 0.64 -2.6 2,673,335 + SGL_RS14340 0.69 -0.3 2,673,335 + SGL_RS14340 0.69 -1.9 2,673,487 - SGL_RS14340 0.77 -0.0 2,673,495 + SGL_RS14340 0.77 -1.4 2,673,542 - SGL_RS14340 0.79 -1.3 2,673,753 - SGL_RS14340 0.89 -1.4 2,673,788 + -3.5 2,673,881 + -0.3 2,673,961 - -0.6 2,673,967 + -1.3 2,673,980 - -0.5 2,673,980 - -0.7 2,674,103 - -0.4 2,674,215 + SGL_RS14345 0.18 -0.4 2,674,334 + SGL_RS14345 0.38 -0.1 2,674,368 + SGL_RS14345 0.43 -0.1 2,674,368 + SGL_RS14345 0.43 -0.2 2,674,376 - SGL_RS14345 0.44 +0.2 2,674,724 - -0.0
Or see this region's nucleotide sequence