Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13555 and SGL_RS13560 are separated by 257 nucleotides SGL_RS13560 and SGL_RS13565 are separated by 241 nucleotides
SGL_RS13555: SGL_RS13555 - aldehyde oxygenase (deformylating), at 2,511,514 to 2,512,209
_RS13555
SGL_RS13560: SGL_RS13560 - glucose-1-phosphate thymidylyltransferase, at 2,512,467 to 2,513,564
_RS13560
SGL_RS13565: SGL_RS13565 - glycine--tRNA ligase subunit beta, at 2,513,806 to 2,515,974
_RS13565
Position (kb)
2512
2513
2514 Strain fitness (log2 ratio)
-2
-1
0
1 at 2511.652 kb on + strand, within SGL_RS13555 at 2511.788 kb on + strand, within SGL_RS13555 at 2512.136 kb on - strand, within SGL_RS13555 at 2512.232 kb on - strand at 2512.377 kb on - strand at 2512.396 kb on - strand at 2512.404 kb on + strand at 2512.407 kb on - strand at 2512.457 kb on - strand at 2512.478 kb on - strand at 2512.494 kb on + strand at 2512.673 kb on - strand, within SGL_RS13560 at 2512.687 kb on - strand, within SGL_RS13560 at 2512.752 kb on + strand, within SGL_RS13560 at 2512.761 kb on + strand, within SGL_RS13560 at 2512.817 kb on + strand, within SGL_RS13560 at 2512.950 kb on + strand, within SGL_RS13560 at 2512.951 kb on - strand, within SGL_RS13560 at 2513.008 kb on + strand, within SGL_RS13560 at 2513.025 kb on + strand, within SGL_RS13560 at 2513.108 kb on - strand, within SGL_RS13560 at 2513.108 kb on - strand, within SGL_RS13560 at 2513.403 kb on + strand, within SGL_RS13560 at 2513.455 kb on - strand at 2513.493 kb on - strand at 2513.514 kb on - strand at 2513.602 kb on - strand at 2513.602 kb on - strand at 2513.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,511,652 + SGL_RS13555 0.20 +0.2 2,511,788 + SGL_RS13555 0.39 +0.5 2,512,136 - SGL_RS13555 0.89 +0.1 2,512,232 - -0.7 2,512,377 - +0.0 2,512,396 - -0.6 2,512,404 + +0.1 2,512,407 - -0.4 2,512,457 - +0.1 2,512,478 - -1.2 2,512,494 + -1.9 2,512,673 - SGL_RS13560 0.19 -1.7 2,512,687 - SGL_RS13560 0.20 -1.1 2,512,752 + SGL_RS13560 0.26 -1.3 2,512,761 + SGL_RS13560 0.27 -1.2 2,512,817 + SGL_RS13560 0.32 -2.1 2,512,950 + SGL_RS13560 0.44 -1.6 2,512,951 - SGL_RS13560 0.44 -2.5 2,513,008 + SGL_RS13560 0.49 -1.9 2,513,025 + SGL_RS13560 0.51 -1.8 2,513,108 - SGL_RS13560 0.58 -0.9 2,513,108 - SGL_RS13560 0.58 -1.7 2,513,403 + SGL_RS13560 0.85 -1.1 2,513,455 - -1.7 2,513,493 - -1.5 2,513,514 - -1.3 2,513,602 - +0.4 2,513,602 - +0.2 2,513,602 - +0.1
Or see this region's nucleotide sequence