Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13360 and SGL_RS13365 are separated by 222 nucleotides SGL_RS13365 and SGL_RS13370 are separated by 66 nucleotides SGL_RS13370 and SGL_RS13375 are separated by 80 nucleotides
SGL_RS13360: SGL_RS13360 - DUF1190 domain-containing protein, at 2,463,555 to 2,464,304
_RS13360
SGL_RS13365: SGL_RS13365 - flavin reductase family protein, at 2,464,527 to 2,465,009
_RS13365
SGL_RS13370: SGL_RS13370 - exonuclease SbcCD subunit D, at 2,465,076 to 2,466,326
_RS13370
SGL_RS13375: SGL_RS13375 - ATP-dependent Clp protease ATP-binding subunit, at 2,466,407 to 2,468,872
_RS13375
Position (kb)
2465
2466
2467 Strain fitness (log2 ratio)
-2
-1
0
1 at 2464.077 kb on - strand, within SGL_RS13360 at 2464.107 kb on + strand, within SGL_RS13360 at 2464.576 kb on + strand, within SGL_RS13365 at 2464.675 kb on - strand, within SGL_RS13365 at 2465.032 kb on + strand at 2465.040 kb on - strand at 2465.098 kb on - strand at 2465.262 kb on + strand, within SGL_RS13370 at 2465.877 kb on + strand, within SGL_RS13370 at 2466.057 kb on + strand, within SGL_RS13370 at 2466.157 kb on + strand, within SGL_RS13370 at 2466.202 kb on + strand at 2466.209 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,464,077 - SGL_RS13360 0.70 +0.2 2,464,107 + SGL_RS13360 0.74 +0.2 2,464,576 + SGL_RS13365 0.10 +0.0 2,464,675 - SGL_RS13365 0.31 -0.8 2,465,032 + -0.2 2,465,040 - +0.1 2,465,098 - -0.3 2,465,262 + SGL_RS13370 0.15 -0.7 2,465,877 + SGL_RS13370 0.64 -2.2 2,466,057 + SGL_RS13370 0.78 -1.8 2,466,157 + SGL_RS13370 0.86 -2.3 2,466,202 + -2.2 2,466,209 + -1.0
Or see this region's nucleotide sequence