Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12685 and SGL_RS12690 overlap by 2 nucleotides SGL_RS12690 and SGL_RS12695 are separated by 227 nucleotides SGL_RS12695 and SGL_RS12700 are separated by 59 nucleotides SGL_RS12700 and SGL_RS12705 overlap by 4 nucleotides
SGL_RS12685: SGL_RS12685 - hypothetical protein, at 2,302,457 to 2,302,639
_RS12685
SGL_RS12690: SGL_RS12690 - hypothetical protein, at 2,302,638 to 2,303,279
_RS12690
SGL_RS12695: SGL_RS12695 - tetratricopeptide repeat protein, at 2,303,507 to 2,304,034
_RS12695
SGL_RS12700: SGL_RS12700 - CBS domain-containing protein, at 2,304,094 to 2,304,561
_RS12700
SGL_RS12705: SGL_RS12705 - recombinase family protein, at 2,304,558 to 2,305,886
_RS12705
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-1
0
1 at 2302.518 kb on + strand, within SGL_RS12685 at 2302.627 kb on - strand at 2302.642 kb on + strand at 2302.678 kb on - strand at 2302.703 kb on - strand, within SGL_RS12690 at 2303.029 kb on + strand, within SGL_RS12690 at 2303.037 kb on - strand, within SGL_RS12690 at 2303.353 kb on + strand at 2303.526 kb on + strand at 2303.881 kb on - strand, within SGL_RS12695 at 2303.944 kb on - strand, within SGL_RS12695 at 2304.270 kb on + strand, within SGL_RS12700 at 2304.857 kb on - strand, within SGL_RS12705 at 2304.935 kb on - strand, within SGL_RS12705 at 2305.000 kb on - strand, within SGL_RS12705
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,302,518 + SGL_RS12685 0.33 -0.0 2,302,627 - +0.0 2,302,642 + +0.1 2,302,678 - +0.4 2,302,703 - SGL_RS12690 0.10 -0.3 2,303,029 + SGL_RS12690 0.61 +0.5 2,303,037 - SGL_RS12690 0.62 +0.4 2,303,353 + -0.2 2,303,526 + +0.4 2,303,881 - SGL_RS12695 0.71 -0.7 2,303,944 - SGL_RS12695 0.83 -1.0 2,304,270 + SGL_RS12700 0.38 +0.9 2,304,857 - SGL_RS12705 0.22 +0.5 2,304,935 - SGL_RS12705 0.28 +0.0 2,305,000 - SGL_RS12705 0.33 +0.2
Or see this region's nucleotide sequence