Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11990 and SGL_RS11995 are separated by 180 nucleotides SGL_RS11995 and SGL_RS12000 are separated by 132 nucleotides SGL_RS12000 and SGL_RS12005 are separated by 187 nucleotides SGL_RS12005 and SGL_RS12010 are separated by 333 nucleotides
SGL_RS11990: SGL_RS11990 - alanine--tRNA ligase, at 2,141,613 to 2,144,246
_RS11990
SGL_RS11995: SGL_RS11995 - hypothetical protein, at 2,144,427 to 2,144,846
_RS11995
SGL_RS12000: SGL_RS12000 - type II CAAX endopeptidase family protein, at 2,144,979 to 2,145,812
_RS12000
SGL_RS12005: SGL_RS12005 - AbrB family transcriptional regulator, at 2,146,000 to 2,146,467
_RS12005
SGL_RS12010: SGL_RS12010 - DUF3038 domain-containing protein, at 2,146,801 to 2,147,376
_RS12010
Position (kb)
2144
2145
2146 Strain fitness (log2 ratio)
-1
0
1 at 2144.314 kb on + strand at 2144.314 kb on + strand at 2144.332 kb on - strand at 2144.359 kb on + strand at 2144.397 kb on - strand at 2144.433 kb on - strand at 2144.433 kb on - strand at 2144.543 kb on - strand, within SGL_RS11995 at 2144.579 kb on + strand, within SGL_RS11995 at 2144.687 kb on - strand, within SGL_RS11995 at 2145.015 kb on - strand at 2145.022 kb on - strand at 2145.147 kb on + strand, within SGL_RS12000 at 2145.174 kb on - strand, within SGL_RS12000 at 2145.210 kb on + strand, within SGL_RS12000 at 2145.218 kb on - strand, within SGL_RS12000 at 2145.218 kb on - strand, within SGL_RS12000 at 2145.264 kb on - strand, within SGL_RS12000 at 2145.336 kb on - strand, within SGL_RS12000 at 2145.388 kb on + strand, within SGL_RS12000 at 2145.465 kb on - strand, within SGL_RS12000 at 2145.522 kb on - strand, within SGL_RS12000 at 2145.706 kb on - strand, within SGL_RS12000 at 2146.683 kb on + strand at 2146.781 kb on + strand at 2146.797 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,144,314 + +0.2 2,144,314 + +0.1 2,144,332 - -0.4 2,144,359 + +0.2 2,144,397 - +0.1 2,144,433 - +0.2 2,144,433 - -0.2 2,144,543 - SGL_RS11995 0.28 +0.4 2,144,579 + SGL_RS11995 0.36 -0.4 2,144,687 - SGL_RS11995 0.62 +0.0 2,145,015 - -0.0 2,145,022 - +0.1 2,145,147 + SGL_RS12000 0.20 -1.4 2,145,174 - SGL_RS12000 0.23 -0.9 2,145,210 + SGL_RS12000 0.28 -1.2 2,145,218 - SGL_RS12000 0.29 -0.8 2,145,218 - SGL_RS12000 0.29 -1.4 2,145,264 - SGL_RS12000 0.34 -1.2 2,145,336 - SGL_RS12000 0.43 +0.0 2,145,388 + SGL_RS12000 0.49 -1.1 2,145,465 - SGL_RS12000 0.58 -1.0 2,145,522 - SGL_RS12000 0.65 -1.6 2,145,706 - SGL_RS12000 0.87 -1.1 2,146,683 + +0.2 2,146,781 + +0.2 2,146,797 - +0.2
Or see this region's nucleotide sequence