Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08700 and SGL_RS08705 are separated by 198 nucleotides SGL_RS08705 and SGL_RS08710 are separated by 248 nucleotides SGL_RS08710 and SGL_RS08715 are separated by 34 nucleotides
SGL_RS08700: SGL_RS08700 - cation-transporting P-type ATPase, at 1,457,213 to 1,459,930
_RS08700
SGL_RS08705: SGL_RS08705 - hypothetical protein, at 1,460,129 to 1,460,566
_RS08705
SGL_RS08710: SGL_RS08710 - folylpolyglutamate synthase/dihydrofolate synthase family protein, at 1,460,815 to 1,462,101
_RS08710
SGL_RS08715: SGL_RS08715 - fatty acid desaturase, at 1,462,136 to 1,463,245
_RS08715
Position (kb)
1460
1461
1462
1463 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1460.536 kb on + strand at 1460.550 kb on + strand at 1460.550 kb on + strand at 1460.784 kb on + strand at 1461.066 kb on - strand, within SGL_RS08710 at 1461.092 kb on + strand, within SGL_RS08710 at 1461.130 kb on + strand, within SGL_RS08710 at 1461.231 kb on + strand, within SGL_RS08710 at 1461.351 kb on - strand, within SGL_RS08710 at 1461.393 kb on + strand, within SGL_RS08710 at 1461.421 kb on - strand, within SGL_RS08710 at 1461.541 kb on + strand, within SGL_RS08710 at 1461.549 kb on - strand, within SGL_RS08710 at 1461.598 kb on - strand, within SGL_RS08710 at 1461.717 kb on - strand, within SGL_RS08710 at 1461.942 kb on + strand, within SGL_RS08710 at 1461.954 kb on - strand, within SGL_RS08710 at 1462.021 kb on + strand at 1462.067 kb on + strand at 1462.210 kb on - strand at 1462.319 kb on - strand, within SGL_RS08715 at 1462.320 kb on - strand, within SGL_RS08715 at 1462.733 kb on + strand, within SGL_RS08715 at 1462.753 kb on - strand, within SGL_RS08715 at 1462.993 kb on + strand, within SGL_RS08715 at 1463.091 kb on + strand, within SGL_RS08715
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,460,536 + +0.1 1,460,550 + -0.2 1,460,550 + -0.3 1,460,784 + -0.0 1,461,066 - SGL_RS08710 0.20 -2.1 1,461,092 + SGL_RS08710 0.22 -0.1 1,461,130 + SGL_RS08710 0.24 -1.3 1,461,231 + SGL_RS08710 0.32 -1.9 1,461,351 - SGL_RS08710 0.42 -1.1 1,461,393 + SGL_RS08710 0.45 -2.4 1,461,421 - SGL_RS08710 0.47 -2.6 1,461,541 + SGL_RS08710 0.56 -0.5 1,461,549 - SGL_RS08710 0.57 -0.8 1,461,598 - SGL_RS08710 0.61 -0.8 1,461,717 - SGL_RS08710 0.70 -3.9 1,461,942 + SGL_RS08710 0.88 -3.6 1,461,954 - SGL_RS08710 0.89 +0.4 1,462,021 + -2.6 1,462,067 + +0.1 1,462,210 - -0.7 1,462,319 - SGL_RS08715 0.16 +0.2 1,462,320 - SGL_RS08715 0.17 +0.1 1,462,733 + SGL_RS08715 0.54 +0.1 1,462,753 - SGL_RS08715 0.56 -0.2 1,462,993 + SGL_RS08715 0.77 -1.1 1,463,091 + SGL_RS08715 0.86 -0.4
Or see this region's nucleotide sequence