Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07715 and SGL_RS07720 are separated by 265 nucleotides SGL_RS07720 and SGL_RS07725 are separated by 57 nucleotides SGL_RS07725 and SGL_RS07730 are separated by 61 nucleotides
SGL_RS07715: SGL_RS07715 - GNAT family N-acetyltransferase, at 1,248,284 to 1,250,176
_RS07715
SGL_RS07720: SGL_RS07720 - phosphate acetyltransferase, at 1,250,442 to 1,252,535
_RS07720
SGL_RS07725: SGL_RS07725 - hypothetical protein, at 1,252,593 to 1,253,207
_RS07725
SGL_RS07730: SGL_RS07730 - DNA/RNA nuclease SfsA, at 1,253,269 to 1,253,982
_RS07730
Position (kb)
1250
1251
1252
1253 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1249.449 kb on + strand, within SGL_RS07715 at 1249.496 kb on - strand, within SGL_RS07715 at 1249.806 kb on + strand, within SGL_RS07715 at 1249.962 kb on + strand, within SGL_RS07715 at 1250.694 kb on - strand, within SGL_RS07720 at 1250.703 kb on - strand, within SGL_RS07720 at 1250.921 kb on - strand, within SGL_RS07720 at 1250.951 kb on + strand, within SGL_RS07720 at 1251.014 kb on + strand, within SGL_RS07720 at 1251.122 kb on - strand, within SGL_RS07720 at 1251.155 kb on - strand, within SGL_RS07720 at 1251.198 kb on - strand, within SGL_RS07720 at 1251.199 kb on - strand, within SGL_RS07720 at 1251.258 kb on + strand, within SGL_RS07720 at 1251.269 kb on - strand, within SGL_RS07720 at 1251.313 kb on + strand, within SGL_RS07720 at 1251.320 kb on + strand, within SGL_RS07720 at 1251.838 kb on + strand, within SGL_RS07720 at 1251.846 kb on - strand, within SGL_RS07720 at 1251.852 kb on - strand, within SGL_RS07720 at 1251.952 kb on + strand, within SGL_RS07720 at 1252.051 kb on - strand, within SGL_RS07720 at 1252.285 kb on - strand, within SGL_RS07720 at 1252.292 kb on + strand, within SGL_RS07720 at 1252.302 kb on - strand, within SGL_RS07720 at 1252.387 kb on + strand at 1252.725 kb on + strand, within SGL_RS07725 at 1252.733 kb on - strand, within SGL_RS07725 at 1252.855 kb on + strand, within SGL_RS07725 at 1252.941 kb on - strand, within SGL_RS07725 at 1252.971 kb on - strand, within SGL_RS07725 at 1253.205 kb on - strand at 1253.283 kb on - strand at 1253.354 kb on + strand, within SGL_RS07730 at 1253.443 kb on + strand, within SGL_RS07730
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,249,449 + SGL_RS07715 0.62 +0.3 1,249,496 - SGL_RS07715 0.64 -0.2 1,249,806 + SGL_RS07715 0.80 +0.1 1,249,962 + SGL_RS07715 0.89 -0.8 1,250,694 - SGL_RS07720 0.12 +0.3 1,250,703 - SGL_RS07720 0.12 +0.5 1,250,921 - SGL_RS07720 0.23 -0.1 1,250,951 + SGL_RS07720 0.24 -0.1 1,251,014 + SGL_RS07720 0.27 +0.1 1,251,122 - SGL_RS07720 0.32 -0.1 1,251,155 - SGL_RS07720 0.34 +0.3 1,251,198 - SGL_RS07720 0.36 -0.5 1,251,199 - SGL_RS07720 0.36 -0.1 1,251,258 + SGL_RS07720 0.39 +0.0 1,251,269 - SGL_RS07720 0.39 -0.3 1,251,313 + SGL_RS07720 0.42 -0.2 1,251,320 + SGL_RS07720 0.42 +0.2 1,251,838 + SGL_RS07720 0.67 -0.4 1,251,846 - SGL_RS07720 0.67 -0.0 1,251,852 - SGL_RS07720 0.67 -0.1 1,251,952 + SGL_RS07720 0.72 -0.3 1,252,051 - SGL_RS07720 0.77 +0.6 1,252,285 - SGL_RS07720 0.88 +0.1 1,252,292 + SGL_RS07720 0.88 +0.0 1,252,302 - SGL_RS07720 0.89 +0.2 1,252,387 + -0.2 1,252,725 + SGL_RS07725 0.21 -0.2 1,252,733 - SGL_RS07725 0.23 -0.2 1,252,855 + SGL_RS07725 0.43 -0.2 1,252,941 - SGL_RS07725 0.57 -3.6 1,252,971 - SGL_RS07725 0.61 -0.2 1,253,205 - -1.1 1,253,283 - +0.3 1,253,354 + SGL_RS07730 0.12 -0.0 1,253,443 + SGL_RS07730 0.24 -0.4
Or see this region's nucleotide sequence