Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07610 and SGL_RS07615 are separated by 39 nucleotides SGL_RS07615 and SGL_RS07620 are separated by 44 nucleotides SGL_RS07620 and SGL_RS07625 are separated by 66 nucleotides
SGL_RS07610: SGL_RS07610 - cyclophane-forming radical SAM/SPASM peptide maturase GrrM/OscB, at 1,229,971 to 1,231,167
_RS07610
SGL_RS07615: SGL_RS07615 - hypothetical protein, at 1,231,207 to 1,231,725
_RS07615
SGL_RS07620: SGL_RS07620 - GrrA/OscA1 family cyclophane-containing rSAM-modified RiPP, at 1,231,770 to 1,232,204
_RS07620
SGL_RS07625: SGL_RS07625 - GrrA/OscA1 family cyclophane-containing rSAM-modified RiPP, at 1,232,271 to 1,232,675
_RS07625
Position (kb) 1231
1232 Strain fitness (log2 ratio) -1
0
1 at 1230.827 kb on - strand, within SGL_RS07610 at 1230.941 kb on - strand, within SGL_RS07610 at 1231.479 kb on + strand, within SGL_RS07615 at 1231.645 kb on + strand, within SGL_RS07615 at 1231.686 kb on - strand at 1231.686 kb on - strand at 1232.066 kb on - strand, within SGL_RS07620 at 1232.198 kb on - strand at 1232.276 kb on - strand at 1232.300 kb on - strand at 1232.555 kb on + strand, within SGL_RS07625 at 1232.576 kb on - strand, within SGL_RS07625 at 1232.725 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,230,827 - SGL_RS07610 0.72 -0.4 1,230,941 - SGL_RS07610 0.81 -0.2 1,231,479 + SGL_RS07615 0.52 -0.2 1,231,645 + SGL_RS07615 0.84 -1.4 1,231,686 - +0.0 1,231,686 - +0.1 1,232,066 - SGL_RS07620 0.68 -0.5 1,232,198 - +0.0 1,232,276 - +0.1 1,232,300 - +0.1 1,232,555 + SGL_RS07625 0.70 -0.4 1,232,576 - SGL_RS07625 0.75 -0.0 1,232,725 + +0.1
Or see this region's nucleotide sequence