Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07300 and SGL_RS07305 are separated by 53 nucleotides SGL_RS07305 and SGL_RS07310 are separated by 157 nucleotides SGL_RS07310 and SGL_RS07315 are separated by 69 nucleotides
SGL_RS07300: SGL_RS07300 - site-2 protease family protein, at 1,161,856 to 1,163,367
_RS07300
SGL_RS07305: SGL_RS07305 - N-acetylmuramic acid 6-phosphate etherase, at 1,163,421 to 1,164,338
_RS07305
SGL_RS07310: SGL_RS07310 - DUF3110 domain-containing protein, at 1,164,496 to 1,165,017
_RS07310
SGL_RS07315: SGL_RS07315 - apocytochrome f, at 1,165,087 to 1,166,073
_RS07315
Position (kb)
1163
1164
1165 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1162.815 kb on - strand, within SGL_RS07300 at 1163.031 kb on - strand, within SGL_RS07300 at 1163.277 kb on - strand at 1163.301 kb on - strand at 1163.328 kb on + strand at 1163.419 kb on + strand at 1163.574 kb on + strand, within SGL_RS07305 at 1163.861 kb on - strand, within SGL_RS07305 at 1163.873 kb on - strand, within SGL_RS07305 at 1163.888 kb on - strand, within SGL_RS07305 at 1163.960 kb on - strand, within SGL_RS07305 at 1163.987 kb on + strand, within SGL_RS07305 at 1164.000 kb on + strand, within SGL_RS07305 at 1164.112 kb on + strand, within SGL_RS07305 at 1164.705 kb on - strand, within SGL_RS07310 at 1164.769 kb on + strand, within SGL_RS07310 at 1164.787 kb on - strand, within SGL_RS07310 at 1164.815 kb on + strand, within SGL_RS07310 at 1164.847 kb on + strand, within SGL_RS07310 at 1164.948 kb on - strand, within SGL_RS07310 at 1164.958 kb on + strand, within SGL_RS07310 at 1164.963 kb on + strand, within SGL_RS07310 at 1165.034 kb on + strand at 1165.040 kb on + strand at 1165.042 kb on - strand at 1165.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,162,815 - SGL_RS07300 0.63 -0.2 1,163,031 - SGL_RS07300 0.78 -0.1 1,163,277 - +0.0 1,163,301 - -1.1 1,163,328 + -0.5 1,163,419 + -0.4 1,163,574 + SGL_RS07305 0.17 -0.3 1,163,861 - SGL_RS07305 0.48 -2.5 1,163,873 - SGL_RS07305 0.49 -2.3 1,163,888 - SGL_RS07305 0.51 -1.5 1,163,960 - SGL_RS07305 0.59 -1.7 1,163,987 + SGL_RS07305 0.62 -2.8 1,164,000 + SGL_RS07305 0.63 -2.0 1,164,112 + SGL_RS07305 0.75 -1.4 1,164,705 - SGL_RS07310 0.40 -0.0 1,164,769 + SGL_RS07310 0.52 -1.0 1,164,787 - SGL_RS07310 0.56 -2.2 1,164,815 + SGL_RS07310 0.61 -1.1 1,164,847 + SGL_RS07310 0.67 -0.9 1,164,948 - SGL_RS07310 0.87 -0.1 1,164,958 + SGL_RS07310 0.89 -0.5 1,164,963 + SGL_RS07310 0.89 -0.3 1,165,034 + +0.9 1,165,040 + -0.1 1,165,042 - -0.1 1,165,048 - -0.5
Or see this region's nucleotide sequence