Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06770 and SGL_RS06775 are separated by 48 nucleotides SGL_RS06775 and SGL_RS06780 overlap by 4 nucleotides SGL_RS06780 and SGL_RS06785 are separated by 500 nucleotides
SGL_RS06770: SGL_RS06770 - cytosine deaminase, at 1,047,005 to 1,048,357
_RS06770
SGL_RS06775: SGL_RS06775 - type II toxin-antitoxin system PemK/MazF family toxin, at 1,048,406 to 1,048,753
_RS06775
SGL_RS06780: SGL_RS06780 - hypothetical protein, at 1,048,750 to 1,049,016
_RS06780
SGL_RS06785: SGL_RS06785 - alpha/beta hydrolase, at 1,049,517 to 1,050,407
_RS06785
Position (kb)
1048
1049 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1047.458 kb on + strand, within SGL_RS06770 at 1047.531 kb on - strand, within SGL_RS06770 at 1047.660 kb on - strand, within SGL_RS06770 at 1047.699 kb on - strand, within SGL_RS06770 at 1047.840 kb on + strand, within SGL_RS06770 at 1047.848 kb on - strand, within SGL_RS06770 at 1047.895 kb on + strand, within SGL_RS06770 at 1047.931 kb on + strand, within SGL_RS06770 at 1048.109 kb on + strand, within SGL_RS06770 at 1048.339 kb on - strand at 1048.403 kb on + strand at 1048.456 kb on - strand, within SGL_RS06775 at 1048.564 kb on - strand, within SGL_RS06775 at 1048.615 kb on + strand, within SGL_RS06775 at 1048.854 kb on + strand, within SGL_RS06780 at 1048.861 kb on + strand, within SGL_RS06780 at 1048.862 kb on - strand, within SGL_RS06780 at 1048.935 kb on + strand, within SGL_RS06780 at 1049.024 kb on - strand at 1049.026 kb on - strand at 1049.212 kb on + strand at 1049.641 kb on - strand, within SGL_RS06785 at 1049.751 kb on + strand, within SGL_RS06785
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 1,047,458 + SGL_RS06770 0.33 -0.2 1,047,531 - SGL_RS06770 0.39 -0.1 1,047,660 - SGL_RS06770 0.48 -0.0 1,047,699 - SGL_RS06770 0.51 -0.3 1,047,840 + SGL_RS06770 0.62 -0.2 1,047,848 - SGL_RS06770 0.62 +0.0 1,047,895 + SGL_RS06770 0.66 -0.8 1,047,931 + SGL_RS06770 0.68 +0.0 1,048,109 + SGL_RS06770 0.82 +0.1 1,048,339 - -1.3 1,048,403 + -0.4 1,048,456 - SGL_RS06775 0.14 -1.1 1,048,564 - SGL_RS06775 0.45 -1.2 1,048,615 + SGL_RS06775 0.60 -3.2 1,048,854 + SGL_RS06780 0.39 -0.8 1,048,861 + SGL_RS06780 0.42 -1.2 1,048,862 - SGL_RS06780 0.42 -0.5 1,048,935 + SGL_RS06780 0.69 -0.9 1,049,024 - -1.0 1,049,026 - -1.3 1,049,212 + -0.5 1,049,641 - SGL_RS06785 0.14 -0.1 1,049,751 + SGL_RS06785 0.26 -0.2
Or see this region's nucleotide sequence