Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05870 and SGL_RS05875 are separated by 91 nucleotides SGL_RS05875 and SGL_RS05880 are separated by 180 nucleotides SGL_RS05880 and SGL_RS05885 are separated by 263 nucleotides
SGL_RS05870: SGL_RS05870 - DUF1823 family protein, at 845,482 to 845,874
_RS05870
SGL_RS05875: SGL_RS05875 - c-type cytochrome, at 845,966 to 846,328
_RS05875
SGL_RS05880: SGL_RS05880 - sugar ABC transporter substrate-binding protein, at 846,509 to 847,810
_RS05880
SGL_RS05885: SGL_RS05885 - acetylglutamate kinase, at 848,074 to 848,967
_RS05885
Position (kb) 846
847
848 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 845.571 kb on - strand, within SGL_RS05870 at 845.669 kb on - strand, within SGL_RS05870 at 845.758 kb on + strand, within SGL_RS05870 at 845.914 kb on + strand at 846.154 kb on + strand, within SGL_RS05875 at 846.369 kb on - strand at 846.391 kb on - strand at 846.779 kb on - strand, within SGL_RS05880 at 846.815 kb on - strand, within SGL_RS05880 at 846.876 kb on + strand, within SGL_RS05880 at 846.876 kb on - strand, within SGL_RS05880 at 847.184 kb on + strand, within SGL_RS05880 at 847.301 kb on - strand, within SGL_RS05880 at 847.690 kb on + strand at 847.741 kb on - strand at 847.969 kb on + strand at 847.980 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 845,571 - SGL_RS05870 0.23 -0.0 845,669 - SGL_RS05870 0.48 +0.5 845,758 + SGL_RS05870 0.70 +0.3 845,914 + +0.2 846,154 + SGL_RS05875 0.52 -0.1 846,369 - -0.0 846,391 - +0.0 846,779 - SGL_RS05880 0.21 -0.7 846,815 - SGL_RS05880 0.24 -2.6 846,876 + SGL_RS05880 0.28 -0.8 846,876 - SGL_RS05880 0.28 -2.6 847,184 + SGL_RS05880 0.52 -0.5 847,301 - SGL_RS05880 0.61 -2.8 847,690 + -1.9 847,741 - -0.5 847,969 + -0.2 847,980 + +1.1
Or see this region's nucleotide sequence