Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04330 and SGL_RS04335 are separated by 28 nucleotides SGL_RS04335 and SGL_RS04340 are separated by 210 nucleotides SGL_RS04340 and SGL_RS04345 are separated by 245 nucleotides
SGL_RS04330: SGL_RS04330 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 529,735 to 530,688
_RS04330
SGL_RS04335: SGL_RS04335 - GNAT family N-acetyltransferase, at 530,717 to 531,235
_RS04335
SGL_RS04340: SGL_RS04340 - type I glutamate--ammonia ligase, at 531,446 to 532,867
_RS04340
SGL_RS04345: SGL_RS04345 - response regulator, at 533,113 to 537,474
_RS04345
Position (kb) 531
532
533 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 530.518 kb on - strand, within SGL_RS04330 at 530.806 kb on + strand, within SGL_RS04335 at 530.806 kb on + strand, within SGL_RS04335 at 530.814 kb on - strand, within SGL_RS04335 at 530.895 kb on + strand, within SGL_RS04335 at 530.966 kb on + strand, within SGL_RS04335 at 530.974 kb on + strand, within SGL_RS04335 at 531.193 kb on - strand at 531.337 kb on - strand at 531.395 kb on + strand at 531.493 kb on + strand at 531.539 kb on - strand at 531.575 kb on + strand at 531.585 kb on - strand at 531.620 kb on - strand, within SGL_RS04340 at 531.664 kb on + strand, within SGL_RS04340 at 531.751 kb on + strand, within SGL_RS04340 at 531.751 kb on + strand, within SGL_RS04340 at 531.759 kb on - strand, within SGL_RS04340 at 531.764 kb on - strand, within SGL_RS04340 at 531.764 kb on - strand, within SGL_RS04340 at 531.779 kb on - strand, within SGL_RS04340 at 531.914 kb on + strand, within SGL_RS04340 at 531.960 kb on - strand, within SGL_RS04340 at 532.006 kb on + strand, within SGL_RS04340 at 532.093 kb on + strand, within SGL_RS04340 at 532.102 kb on - strand, within SGL_RS04340 at 532.281 kb on + strand, within SGL_RS04340 at 532.389 kb on - strand, within SGL_RS04340 at 532.436 kb on - strand, within SGL_RS04340 at 532.457 kb on + strand, within SGL_RS04340 at 532.515 kb on - strand, within SGL_RS04340 at 532.547 kb on + strand, within SGL_RS04340 at 532.584 kb on + strand, within SGL_RS04340 at 532.628 kb on + strand, within SGL_RS04340 at 532.715 kb on - strand, within SGL_RS04340 at 532.756 kb on + strand at 532.793 kb on + strand at 532.821 kb on - strand at 532.903 kb on - strand at 533.168 kb on + strand at 533.176 kb on - strand at 533.199 kb on - strand at 533.483 kb on + strand at 533.507 kb on - strand at 533.521 kb on + strand at 533.574 kb on - strand, within SGL_RS04345 at 533.614 kb on - strand, within SGL_RS04345 at 533.639 kb on + strand, within SGL_RS04345 at 533.715 kb on + strand, within SGL_RS04345 at 533.723 kb on - strand, within SGL_RS04345
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 530,518 - SGL_RS04330 0.82 +0.3 530,806 + SGL_RS04335 0.17 +0.1 530,806 + SGL_RS04335 0.17 -0.1 530,814 - SGL_RS04335 0.19 -1.0 530,895 + SGL_RS04335 0.34 -0.9 530,966 + SGL_RS04335 0.48 -0.1 530,974 + SGL_RS04335 0.50 -0.8 531,193 - -0.5 531,337 - +0.1 531,395 + -3.1 531,493 + -2.7 531,539 - -4.3 531,575 + -4.0 531,585 - -2.7 531,620 - SGL_RS04340 0.12 -3.6 531,664 + SGL_RS04340 0.15 -3.4 531,751 + SGL_RS04340 0.21 -3.9 531,751 + SGL_RS04340 0.21 -2.0 531,759 - SGL_RS04340 0.22 -3.7 531,764 - SGL_RS04340 0.22 -2.0 531,764 - SGL_RS04340 0.22 -2.0 531,779 - SGL_RS04340 0.23 -1.2 531,914 + SGL_RS04340 0.33 -3.2 531,960 - SGL_RS04340 0.36 -1.8 532,006 + SGL_RS04340 0.39 +1.6 532,093 + SGL_RS04340 0.45 -3.3 532,102 - SGL_RS04340 0.46 -3.2 532,281 + SGL_RS04340 0.59 -4.8 532,389 - SGL_RS04340 0.66 -2.5 532,436 - SGL_RS04340 0.70 -2.9 532,457 + SGL_RS04340 0.71 -4.7 532,515 - SGL_RS04340 0.75 -3.0 532,547 + SGL_RS04340 0.77 -1.7 532,584 + SGL_RS04340 0.80 -1.4 532,628 + SGL_RS04340 0.83 -2.9 532,715 - SGL_RS04340 0.89 -3.4 532,756 + -2.8 532,793 + -4.6 532,821 - -2.9 532,903 - -0.2 533,168 + -0.7 533,176 - -0.4 533,199 - -0.4 533,483 + -0.0 533,507 - -0.3 533,521 + -0.0 533,574 - SGL_RS04345 0.11 +0.2 533,614 - SGL_RS04345 0.11 -0.4 533,639 + SGL_RS04345 0.12 -0.5 533,715 + SGL_RS04345 0.14 -0.7 533,723 - SGL_RS04345 0.14 -0.7
Or see this region's nucleotide sequence