Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03515 and SGL_RS03520 are separated by 38 nucleotides SGL_RS03520 and SGL_RS03525 are separated by 180 nucleotides SGL_RS03525 and SGL_RS03530 are separated by 119 nucleotides
SGL_RS03515: SGL_RS03515 - Uma2 family endonuclease, at 348,701 to 349,429
_RS03515
SGL_RS03520: SGL_RS03520 - ABC transporter permease, at 349,468 to 350,298
_RS03520
SGL_RS03525: SGL_RS03525 - hypothetical protein, at 350,479 to 350,916
_RS03525
SGL_RS03530: SGL_RS03530 - DUF29 domain-containing protein, at 351,036 to 351,467
_RS03530
Position (kb) 349
350
351 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 348.595 kb on + strand at 348.907 kb on - strand, within SGL_RS03515 at 348.945 kb on - strand, within SGL_RS03515 at 349.006 kb on + strand, within SGL_RS03515 at 349.197 kb on - strand, within SGL_RS03515 at 349.278 kb on - strand, within SGL_RS03515 at 349.308 kb on + strand, within SGL_RS03515 at 349.329 kb on + strand, within SGL_RS03515 at 349.489 kb on - strand at 349.541 kb on - strand at 349.562 kb on - strand, within SGL_RS03520 at 349.644 kb on - strand, within SGL_RS03520 at 349.674 kb on + strand, within SGL_RS03520 at 349.831 kb on - strand, within SGL_RS03520 at 349.987 kb on + strand, within SGL_RS03520 at 350.454 kb on - strand at 350.454 kb on - strand at 350.584 kb on + strand, within SGL_RS03525 at 350.598 kb on - strand, within SGL_RS03525 at 350.731 kb on + strand, within SGL_RS03525 at 350.761 kb on + strand, within SGL_RS03525 at 350.871 kb on - strand, within SGL_RS03525 at 351.239 kb on - strand, within SGL_RS03530
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 348,595 + +0.0 348,907 - SGL_RS03515 0.28 -0.4 348,945 - SGL_RS03515 0.33 +0.3 349,006 + SGL_RS03515 0.42 -0.2 349,197 - SGL_RS03515 0.68 -0.4 349,278 - SGL_RS03515 0.79 +0.1 349,308 + SGL_RS03515 0.83 -0.3 349,329 + SGL_RS03515 0.86 +0.1 349,489 - -2.1 349,541 - -2.4 349,562 - SGL_RS03520 0.11 -3.3 349,644 - SGL_RS03520 0.21 -4.5 349,674 + SGL_RS03520 0.25 -1.4 349,831 - SGL_RS03520 0.44 -2.4 349,987 + SGL_RS03520 0.62 -1.2 350,454 - +0.3 350,454 - +0.0 350,584 + SGL_RS03525 0.24 +0.3 350,598 - SGL_RS03525 0.27 -0.1 350,731 + SGL_RS03525 0.58 -0.2 350,761 + SGL_RS03525 0.64 +0.7 350,871 - SGL_RS03525 0.89 -0.2 351,239 - SGL_RS03530 0.47 -0.3
Or see this region's nucleotide sequence