Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03420 and SGL_RS03425 are separated by 83 nucleotides SGL_RS03425 and SGL_RS03430 are separated by 102 nucleotides
SGL_RS03420: SGL_RS03420 - UbiD family decarboxylase, at 329,555 to 331,060
_RS03420
SGL_RS03425: SGL_RS03425 - precorrin-3B C(17)-methyltransferase, at 331,144 to 333,027
_RS03425
SGL_RS03430: SGL_RS03430 - DUF4335 domain-containing protein, at 333,130 to 334,485
_RS03430
Position (kb) 331
332
333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 330.330 kb on - strand, within SGL_RS03420 at 330.330 kb on - strand, within SGL_RS03420 at 330.547 kb on + strand, within SGL_RS03420 at 330.555 kb on - strand, within SGL_RS03420 at 330.783 kb on - strand, within SGL_RS03420 at 330.870 kb on - strand, within SGL_RS03420 at 330.900 kb on - strand, within SGL_RS03420 at 330.946 kb on - strand at 330.988 kb on + strand at 331.058 kb on - strand at 331.063 kb on - strand at 331.075 kb on - strand at 331.077 kb on + strand at 331.620 kb on - strand, within SGL_RS03425 at 331.870 kb on + strand, within SGL_RS03425 at 331.980 kb on - strand, within SGL_RS03425 at 332.228 kb on - strand, within SGL_RS03425 at 332.255 kb on - strand, within SGL_RS03425 at 332.618 kb on + strand, within SGL_RS03425 at 332.764 kb on + strand, within SGL_RS03425 at 332.775 kb on - strand, within SGL_RS03425 at 333.061 kb on - strand at 333.167 kb on - strand at 333.182 kb on - strand at 333.190 kb on - strand at 333.606 kb on + strand, within SGL_RS03430 at 333.874 kb on - strand, within SGL_RS03430 at 334.020 kb on + strand, within SGL_RS03430
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 330,330 - SGL_RS03420 0.51 -2.1 330,330 - SGL_RS03420 0.51 -1.9 330,547 + SGL_RS03420 0.66 -1.8 330,555 - SGL_RS03420 0.66 -0.8 330,783 - SGL_RS03420 0.82 -2.6 330,870 - SGL_RS03420 0.87 -2.6 330,900 - SGL_RS03420 0.89 -2.4 330,946 - -1.5 330,988 + -3.0 331,058 - -0.6 331,063 - -0.2 331,075 - -2.1 331,077 + -1.4 331,620 - SGL_RS03425 0.25 -1.7 331,870 + SGL_RS03425 0.39 -1.4 331,980 - SGL_RS03425 0.44 -1.6 332,228 - SGL_RS03425 0.58 -1.6 332,255 - SGL_RS03425 0.59 -3.1 332,618 + SGL_RS03425 0.78 -2.6 332,764 + SGL_RS03425 0.86 -3.0 332,775 - SGL_RS03425 0.87 -1.0 333,061 - +0.0 333,167 - +0.3 333,182 - -1.3 333,190 - -0.5 333,606 + SGL_RS03430 0.35 +0.1 333,874 - SGL_RS03430 0.55 +0.0 334,020 + SGL_RS03430 0.66 -0.3
Or see this region's nucleotide sequence