Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03260 and SGL_RS03265 are separated by 149 nucleotides SGL_RS03265 and SGL_RS03270 are separated by 194 nucleotides SGL_RS03270 and SGL_RS20110 are separated by 245 nucleotides
SGL_RS03260: SGL_RS03260 - FUSC family protein, at 295,076 to 297,343
_RS03260
SGL_RS03265: SGL_RS03265 - photosystem II complex extrinsic protein PsbU, at 297,493 to 297,888
_RS03265
SGL_RS03270: SGL_RS03270 - HNH endonuclease, at 298,083 to 298,580
_RS03270
SGL_RS20110: SGL_RS20110 - hypothetical protein, at 298,826 to 298,957
_RS20110
Position (kb)
297
298 Strain fitness (log2 ratio)
-1
0
1 at 296.549 kb on - strand, within SGL_RS03260 at 296.711 kb on + strand, within SGL_RS03260 at 296.719 kb on - strand, within SGL_RS03260 at 296.719 kb on - strand, within SGL_RS03260 at 296.881 kb on + strand, within SGL_RS03260 at 297.288 kb on - strand at 297.780 kb on - strand, within SGL_RS03265 at 297.906 kb on - strand at 298.312 kb on - strand, within SGL_RS03270 at 298.386 kb on + strand, within SGL_RS03270 at 298.495 kb on + strand, within SGL_RS03270 at 298.536 kb on + strand at 298.870 kb on + strand, within SGL_RS20110
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 296,549 - SGL_RS03260 0.65 +0.2 296,711 + SGL_RS03260 0.72 +0.2 296,719 - SGL_RS03260 0.72 -0.3 296,719 - SGL_RS03260 0.72 +0.1 296,881 + SGL_RS03260 0.80 +0.1 297,288 - +0.1 297,780 - SGL_RS03265 0.72 -1.6 297,906 - -1.1 298,312 - SGL_RS03270 0.46 +0.0 298,386 + SGL_RS03270 0.61 -0.0 298,495 + SGL_RS03270 0.83 -0.1 298,536 + -1.1 298,870 + SGL_RS20110 0.33 +0.5
Or see this region's nucleotide sequence